Instillation of liposomes vs dimethyl sulphoxide or pentosan polysulphate for reducing bladder hyperactivity
Instillation of liposomes vs dimethyl sulphoxide or pentosan polysulphate for reducing bladder hyperactivity
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DOI：
10.1111/j.1464-410x.2009.08673.x
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发表时间：
2009-12-01
影响因子：
4.5
通讯作者：
Chancellor, Michael B.
中科院分区：
文献类型：
--
作者：
Tyagi, Pradeep;Hsieh, Vincent C.;Chancellor, Michael B.
目的研究膀胱内脂质体对二甲基亚砜（DMSO）和戊聚糖多硫酸酯（PPS）在减少化学诱导的大鼠膀胱过度活动中的功效。材料和方法在乌拉坦麻醉（1.0 g/kg）下通过连续膀胱测压法评价雌性Sprague-Dawley大鼠的膀胱反射活动。用生理盐水（0.04 mL/min）持续2 h获得对照膀胱测压图（CMG）后，通过1 h输注硫酸鱼精蛋白（10 mg/mL），随后1 h输注KCl（500 mm）诱导膀胱过度活动。然后将6只大鼠分别输注含50% DMSO、PPS（6 mg/mL）或脂质体（2 mg/mL）的KCl制剂2 h。测定收缩间期（ICI）、压力阈值（PT）和基线压力（BP），并观察硫酸鱼精蛋白/氯化钾（鱼精蛋白/氯化钾）序贯灌注诱发膀胱过度活动症的疗效。输注PPS（增加58.1%）和脂质体（增加156.8%）后ICI显著增加，但DMSO未增加。脂质体输注对PT无显著影响，但PPS组PT略有增加（增加12.4%）。有一个大的和显着的增加PT和BP与DMSO（增加116.5%）和BP基本保持不变后滴注脂质体或PPS. CONCLUSIONSIntravesive脂质体和PPS有一个有益的效果，在膀胱过度活动大鼠模型，而急性滴注DMSO没有。与PPS相比，膀胱灌注脂质体可有效地使ICI增加一倍，可能成为膀胱过度活动症的一种新的治疗选择。
OBJECTIVETo investigate the efficacy of intravesical liposomes against dimethyl sulphoxide (DMSO), and pentosan polysulphate (PPS) in reducing chemically induced bladder hyperactivity in rats.MATERIALS AND METHODSBladder reflex activity of female Sprague-Dawley rats was evaluated by continuous cystometry under urethane anaesthesia (1.0 g/kg). After obtaining a control cystometrogram (CMG) with normal saline (0.04 mL/min) for 2 h, bladder hyperactivity was then induced by 1 h infusion of protamine sulphate (10 mg/mL) followed by a 1-h infusion of KCl (500 mm). Six rats each were then infused with KCl-based preparations containing either 50% DMSO, PPS (6 mg/mL), or liposomes (2 mg/mL) for 2 h. The variables measured included the intercontraction interval (ICI), pressure threshold (PT) and baseline pressure (BP).RESULTSSequential infusion of protamine sulphate/KCl induced hyperactive bladder with no significant difference in ICI, PT or BP among groups before initiating treatment. ICI was significantly increased after infusion of PPS (58.1% increase) and liposomes (156.8% increase) but there was no increase with DMSO. PT was not significantly affected by liposome infusion but slightly increased with PPS (12.4% increase). There was a large and significant increase in PT and BP with DMSO (116.5% increase) and BP largely remained unchanged after instillation with liposomes or PPS.CONCLUSIONSIntravesical liposomes and PPS have a beneficial effect in a bladder hyperactivity rat model, while acute instillation of DMSO does not. Intravesical liposomes were effective in doubling the ICI compared with PPS, and might be a new treatment option for bladder hyperactivity.