Interleukin-11 receptor expression in primary ovarian carcinomas
Interleukin-11 receptor expression in primary ovarian carcinomas
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DOI：
10.1006/gyno.2000.6064
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发表时间：
2001-02-01
影响因子：
4.7
通讯作者：
Savarese, TM
中科院分区：
文献类型：
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作者：
Campbell, CL;Guardiani, R;Savarese, TM
目标。本研究的目的是确定(1)白细胞介素-11受体α亚基（IL-11R α）及其信号转导亚基gp130在原发性卵巢癌中的表达频率；(2) IL-11在卵巢癌中的表达频率；(3) IL-11在卵巢癌细胞生物学中的潜在作用。采用免疫组化法检测IL-11Ra和gp130辅助因子在原发性卵巢癌中的表达；应用逆转录聚合酶链式反应（RT-PCR）检测IL-11在卵巢恶性肿瘤中的表达，并利用ELISA法定量研究IL-11在表达il - 11r的卵巢癌细胞株（OVCAR-3和SKOV-3）中刺激[H-3]胸腺嘧啶结合和/或消除凋亡诱导剂介导的细胞死亡的能力。IL-11R α在48例原发性卵巢癌样本中的45例（93.8%）的恶性上皮细胞中表达。在45例原发性卵巢癌样本中，检测了IL-11受体（IL-11R α和gp130）的两种成分，其中42例（93.3%）观察到共表达。IL-11受体成分在细胞间质层也有表达。IL-11Ra和gp130的共表达在良性卵巢肿瘤和正常卵巢上皮中均可见。相比之下，IL-11 mRNA仅在21个恶性肿瘤样本中表达（14.3%）。在OVCAR -3或SKOV-3细胞的体外实验中，重组人IL-11既不能刺激[H-3]胸苷结合，也不能阻断紫杉醇或fas活化抗体引起的细胞死亡。IL-11受体系统在卵巢恶性和非恶性组织中均有表达，但其在卵巢上皮细胞生物学中的功能尚不清楚。(C) 2001学术出版社。
Objectives. The objectives of this study were to determine (1) the frequency of expression of the interleukin-11 receptor alpha subunit (IL-11R alpha) and its signal transducing subunit, gp130, among primary ovarian carcinomas; (2) the frequency of expression of IL-11 in ovarian carcinomas; and (3) the potential role IL-11 might have in ovarian cancer cell biology.Methods. An immunohistochemical assay was used to determine the expression of IL-11Ra and the gp130 cofactor among primary ovarian carcinomas; the expression of IL-11 in ovarian malignancies was determined using reverse transcription polymerase chain reaction (RT-PCR), The ability of IL-11 to stimulate [H-3]thymidine incorporation in IL-11R-expressing ovarian carcinoma cell lines (OVCAR-3 and SKOV-3) and/or abrogate cell death mediated by apoptosis-inducing agents using an ELISA assay that quantitates DNA fragmentation was also studied.Results. IL-11R alpha was expressed in the malignant epithelial cells of 45 of 48 (93.8%) primary ovarian carcinoma samples studied. In 45 primary ovarian carcinoma samples where both components of the IL-11 receptor (IL-11R alpha and gp130) were examined, coexpression was observed in 42 (93.3%). Expression of the IL-11 receptor components was also found in the stromal layer. Coexpression of IL-11Ra and gp130 was commonly observed in both benign ovarian tumors and in the epithelial layer of normal ovaries. In contrast, IL-11 mRNA was expressed in only 3 of 21 malignant samples studied (14.3%). Recombinant human IL-11 was unable either to stimulate [H-3]thymidine incorporation or to block cell death effected by paclitaxel or Fas-activating antibodies in in vitro assays using OVCAR -3 or SKOV-3 cells.Conclusions. The IL-11 receptor system is commonly expressed in both malignant and nonmalignant ovarian tissues, although its function in ovarian epithelial cell biology remains unclear. (C) 2001 Academic Press.