Myeloid-derived suppressor cell (MDSC) key genes analysis in rat anti-CD28-induced immune tolerance kidney transplantation.
Myeloid-derived suppressor cell (MDSC) key genes analysis in rat anti-CD28-induced immune tolerance kidney transplantation.
复制标题
大鼠抗CD28诱导免疫耐受肾移植中髓系抑制细胞（MDSC）关键基因分析
DOI：
10.21037/tau-20-943
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发表时间：
2021-01
影响因子：
2
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Yang T;Li J;Jia Y;Yang C;Sang R;Zhu T;Xu M;Rong R;Yang C
在移植领域，诱导受体免疫耐受具有重要意义。用抗CD28抗体阻断共刺激分子可以诱导大鼠肾移植模型的耐受。髓系来源的抑制细胞(MDSCs)在肾移植中显示出强大的免疫抑制能力。在这个模型中，我们分析了导致移植耐受的MDSCs的关键基因。对基因表达总表(GEO)数据库中登录号为GSE28545的大鼠基因表达谱芯片数据进行分析。用R语言运行LIMMA程序包，找到差异表达基因(Deg)。在注释、可视化和综合发现数据库(David)中对DEGS进行了浓缩分析，以探索基因本体论(GO)注释及其京都基因和基因组百科全书(KEGG)途径。它们的蛋白质-蛋白质相互作用(PPI)由字符串数据库提供，并在细胞景观中可视化。Hub基因由CytoHubba执行。共输出基因338个，其中上调基因27个，下调基因311个。评估了DEGS的功能和KEGG通路，并基于DEGS的串相互作用构建了PPI网络。整个PPI网络由192个节点和469条边组成。HUB基因包括ZAP70、CDC42、STAT1、STAT4、CCL5和CXCR3。这些关键基因和相应的蛋白及其功能可能为基础和临床研究提供有价值的背景，并可能成为未来免疫耐受研究的方向，特别是那些检测免疫细胞诱导耐受的研究。
In the field of transplantation, inducing immune tolerance in recipients is of great importance. Blocking co-stimulatory molecule using anti-CD28 antibody could induce tolerance in a rat kidney transplantation model. Myeloid-derived suppressor cells (MDSCs) reveals strong immune suppressive abilities in kidney transplantation. Here we analyzed key genes of MDSCs leading to transplant tolerance in this model. Microarray data of rat gene expression profiles under accession number GSE28545 in the Gene Expression Omnibus (GEO) database were analyzed. Running the LIMMA package in R language, the differentially expressed genes (DEGs) were found. Enrichment analysis of the DEGs was conducted in the Database for Annotation, Visualization and Integrated Discovery (DAVID) database to explore gene ontology (GO) annotation and their Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Their protein-protein interactions (PPIs) were provided by STRING database and was visualized in Cytoscape. Hub genes were carried out by CytoHubba. Three hundred and thirty-eight DEGs were exported, including 27 upregulated and 311 downregulated genes. The functions and KEGG pathways of the DEGs were assessed and the PPI network was constructed based on the string interactions of the DEGs. The network was visualized in Cytoscape; the entire PPI network consisted of 192 nodes and 469 edges. Zap70, Cdc42, Stat1, Stat4, Ccl5 and Cxcr3 were among the hub genes. These key genes, corresponding proteins and their functions may provide valuable background for both basic and clinical research and could be the direction of future studies in immune tolerance, especially those examining immunocyte-induced tolerance.
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影响因子：
14.9
作者：
Ritchie ME;Phipson B;Wu D;Hu Y;Law CW;Shi W;Smyth GK
通讯作者：
Smyth GK
影响因子：
17.1
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Morris H;DeWolf S;Robins H;Sprangers B;LoCascio SA;Shonts BA;Kawai T;Wong W;Yang S;Zuber J;Shen Y;Sykes M
通讯作者：
Sykes M
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82.9
作者：
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通讯作者：
Gabrilovich, Dmitry I.
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4.4
作者：
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通讯作者：
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影响因子：
8.8
作者：
Poirier, N.;Mary, C.;Vanhove, B.
通讯作者：
Vanhove, B.