Sera from children with active Henoch-Schonlein purpura can enhance the production of interleukin 8 by human umbilical venous endothelial cells
Sera from children with active Henoch-Schonlein purpura can enhance the production of interleukin 8 by human umbilical venous endothelial cells
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DOI：
10.1136/ard.2003.016196
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发表时间：
2004-11-01
影响因子：
27.4
通讯作者：
Chiang, BL
中科院分区：
文献类型：
--
作者：
Yang, YH;Lai, HJ;Chiang, BL
目的：探讨活动性过敏性紫癜（HSP）患儿血清对内皮细胞白细胞介素（IL） 8生成及细胞间粘附分子(ICAM)-1表达的影响。方法：选取9例活动性HSP患儿和9例正常健康患儿。分析不同阶段患者及对照组血清il - 8水平。检测人脐静脉内皮细胞il - 8的产生和ICAM-1的表达（ELISA法检测il - 8，流式细胞术检测ICAM-1），并在不同刺激条件下进行比较，包括不同阶段患者血清、对照组血清和单独培养基。结果：急性期血清il - 8水平升高。人脐静脉内皮细胞（HUVEC）与活动性HSP患儿血清共培养的上清液中il - 8水平显著高于未处理的HUVEC （p = 0.001）、与活动性血清处理的HUVEC （p = 0.004）和与健康对照血清处理的HUVEC （p = 0.004）。患者和对照组血清均未增强HUVEC上ICAM-1的表达。结论：活动性热休克儿童血清中可能存在一些因子，可以激活内皮细胞产生il - 8。这一过程可能部分解释了热休克中血管周围中性粒细胞浸润和白细胞增多的机制。
Objective: To investigate the ability of sera from children with active Henoch-Schonlein purpura (HSP) to enhance endothelial interleukin (IL) 8 production and intercellular adhesion molecule (ICAM)-1 expression.Methods: Nine children with active HSP and nine normal healthy children were enrolled. IL8 serum levels of patients and controls at different stages were analysed. Production of IL8 and expression of ICAM-1 by human umbilical venous endothelial cells were detected (ELISA for IL8, flow cytometry for ICAM-1) and compared under various stimuli, including sera of patients at different stages, sera of controls, and medium alone.Results: Serum levels of IL8 were increased at the acute stage. Levels of IL8 in supernatants from human umbilical venous endothelial cells (HUVEC) co-cultured with sera from children with active HSP were significantly higher than those from HUVEC without any treatment (p = 0.001), HUVEC treated with inactive sera (p = 0.004), and HUVEC treated with sera from healthy controls (p = 0.004). Sera from patients and from controls did not enhance the expression of ICAM-1 on HUVEC.Conclusions: Some factors may be present in sera from children with active HSP that could activate endothelial cells to produce IL8. This process may account, in part, for the mechanisms of perivascular neutrophil infiltration and leucocytosis in HSP.