CONTROL OF SV40 TRANSCRIPTION DURING A LYTIC INFECTION - LATE RNA-SYNTHESIS IN PRESENCE OF INHIBITORS OF DNA-REPLICATION
CONTROL OF SV40 TRANSCRIPTION DURING A LYTIC INFECTION - LATE RNA-SYNTHESIS IN PRESENCE OF INHIBITORS OF DNA-REPLICATION
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DOI：
10.1093/nar/4.3.551
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发表时间：
1977-01-01
影响因子：
14.9
通讯作者：
BROWN，M
中科院分区：
文献类型：
--
作者：
ROSENTHAL，LJ;BROWN，M
使用DNA复制抑制剂和SV 40的早期（A组）温度敏感（ts）突变体分析了在生产性感染的BSC-1细胞中SV 40 DNA从早期转录到晚期转录的转变。在存在三种DNA复制抑制剂（Ara-C、FdU和氯喹）的感染培养物中，检测到在中性蔗糖梯度中16 S处沉降的晚期病毒特异性胞质RNA，并与SV 40的正（L）DNA链互补，尽管病毒DNA复制的抑制似乎基本上是完全的。在用早期SV 40突变体tsA 58感染后，在限制性温度（41°C）下没有检测到DNA复制，并且在感染细胞的细胞质或细胞核中没有发现与正（L）链互补的显著晚期RNA。这些数据支持晚期病毒功能的表达需要病毒DNA合成或功能基因A蛋白或两者的起始的概念。
The transition from early to late transcription of SV40 DNA in productively infected BSC-1 cells was analyzed using both inhibitors of DNA replication, and early (Group A) temperature sensitive (ts) mutants of SV40. Late virus-specific cytoplasmic RNA sedimenting at 16S in neutral sucrose gradients and complementary to the plus (L) DNA strand of SV40 was detected in cultures infected in the presence of three inhibitors of DNA replication (Ara-C, FdU, and chloroquine), even though the inhibition of viral DNA replication appeared to be essentially complete. After infection with the early SV40 mutant tsA58, no DNA replication was detected at the restrictive temperature (41°C) and no significant late RNA complementary to the plus (L) strand was found, in either the cytoplasm or nuclei of infected cells. These data support the concept that expression of late viral functions requires the initiation of viral DNA synthesis or a functional gene A protein, or both.