Improvements to bead-based oligonucleotide ligation SNP genotyping assays.
Improvements to bead-based oligonucleotide ligation SNP genotyping assays.
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DOI：
10.2144/000112960
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发表时间：
2008-11
期刊：
影响因子：
2.7
通讯作者：
Brzustowicz L
中科院分区：
文献类型：
--
作者：
Bruse S;Moreau M;Azaro M;Zimmerman R;Brzustowicz L
我们描述了一种基于微珠的多重寡核苷酸连接试验（奥拉）在Luminex流式细胞仪上进行。这种方法与以前报道的方法之间的差异包括使用更少的珠子和使用通用寡核苷酸进行信号检测。这些创新有助于显著降低分析成本，同时保持稳健性和准确性。在Luminex奥拉与焦磷酸测序和直接测序之间进行比较。实验，以评估转换率，调用率和一致性的技术复制。
We describe a bead-based, multiplexed, oligonucleotide ligation assay (OLA) performed on the Luminex flow cytometer. Differences between this method and those previously reported include the use of far fewer beads and the use of a universal oligonucleotide for signal detection. These innovations serve to significantly reduce the cost of the assay, while maintaining robustness and accuracy. Comparisons are made between the Luminex OLA and both pyrosequencing and direct sequencing. Experiments to assess conversion rates, call rates, and concordance across technical replicates are also presented.