An Extracellular Serine/Threonine-Rich Protein from Lactobacillus plantarum NCIMB 8826 Is a Novel Aggregation-Promoting Factor with Affinity to Mucin
An Extracellular Serine/Threonine-Rich Protein from Lactobacillus plantarum NCIMB 8826 Is a Novel Aggregation-Promoting Factor with Affinity to Mucin
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DOI：
10.1128/aem.01657-13
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发表时间：
2013-10-01
影响因子：
4.4
通讯作者：
Sanchez, Borja
中科院分区：
文献类型：
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作者：
Hevia, Arancha;Martinez, Noelia;Sanchez, Borja
乳酸菌中的自聚集与某些细胞外蛋白质的产生直接相关，特别是促聚集因子（APF）。聚集促进因子的产生赋予益生菌生产菌株有益的特性，有助于它们适应肠道环境。此外，已提出与病原体的共聚集是益生乳酸菌中的有益机制。这种机制将限制病原体附着在肠道粘膜上，有利于人体免疫系统将其清除。在本文中，我们已经确定了一种新的聚集促进因子的植物乳杆菌。D1基因敲除的突变体显示其自身聚集表型的丧失和与粘蛋白结合的能力降低，表明该蛋白的粘附作用。此外，异源生产的D1蛋白或蛋白质的内部片段，其特征在于其丰富的丝氨酸/苏氨酸，强烈诱导乳酸乳球菌中的自聚集。这一结果强烈表明该内部片段负责D1作为APF的生物活性。据我们所知，这是第一个报告的基因编码的聚集促进因子在Lb。植物
Autoaggregation in lactic acid bacteria is directly related to the production of certain extracellular proteins, notably, aggregation-promoting factors (APFs). Production of aggregation-promoting factors confers beneficial traits to probiotic-producing strains, contributing to their fitness for the intestinal environment. Furthermore, coaggregation with pathogens has been proposed to be a beneficial mechanism in probiotic lactic acid bacteria. This mechanism would limit attachment of the pathogen to the gut mucosa, favoring its removal by the human immune system. In the present paper, we have characterized a novel aggregation-promoting factor in Lactobacillus plantarum. A mutant with a knockout of the D1 gene showed loss of its autoaggregative phenotype and a decreased ability to bind to mucin, indicating an adhesion role of this protein. In addition, heterologous production of the D1 protein or an internal fragment of the protein, characterized by its abundance in serine/threonine, strongly induced autoaggregation in Lactococcus lactis. This result strongly suggested that this internal fragment is responsible for the bioactivity of D1 as an APF. To our knowledge, this is the first report on a gene coding for an aggregation-promoting factor in Lb. plantarum.