Structural characterization of the O-polysaccharide of the lipopolysaccharide produced by Salmonella milwaukee O:43 (group U) which possesses human blood group B activity.
Structural characterization of the O-polysaccharide of the lipopolysaccharide produced by Salmonella milwaukee O:43 (group U) which possesses human blood group B activity.
复制标题
具有人类 B 型血活性的密尔沃基沙门氏菌 O:43（U 组）产生的脂多糖的 O-多糖的结构特征。
DOI：
10.1139/o92-007
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
L. Maclean
中科院分区：
文献类型：
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作者：
M. Perry;L. Maclean
由沙门氏菌密尔沃基O：43（U组）产生的脂多糖的O-多糖通过组成分析、甲基化、高碘酸盐氧化以及1H和13 C核磁共振光谱分析方法显示为具有以下结构的支链五糖重复单元的聚合物：[式：O-多糖的血型活性通过其与人血型B的特异性单克隆抗体的血清学反应性来确定，使用被动血凝和ELISA测定，表明共同抗原表位是由L-Fucp和D-Galp（1：2）残基组成的非还原性末端三糖单元。
The O-polysaccharide of the lipopolysaccharide produced by Salmonella milwaukee O:43 (group U) was shown by composition analysis, methylation, periodate oxidation, and 1H and 13C nuclear magnetic resonance spectroscopic analytical methods to be a polymer of branched pentasaccharide repeating units having the structure: [formula: see text] The blood-group activity of the O-polysaccharide was established by its serological reactivity with a specific monoclonal antibody to human blood group B, using passive hemagglutination and ELISA assays, indicating the common antigenic epitope to be a nonreducing terminal trisaccharide unit composed of L-Fucp and D-Galp (1:2) residues.