Erratum: Weibel-Palade body size modulates the adhesive activity of its von Willebrand Factor cargo in cultured endothelial cells.
Erratum: Weibel-Palade body size modulates the adhesive activity of its von Willebrand Factor cargo in cultured endothelial cells.
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DOI：
10.1038/srep33938
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发表时间：
2016-09-26
影响因子：
4.6
通讯作者：
Cutler DF
中科院分区：
文献类型：
--
作者：
Ferraro F;da Silva ML;Grimes W;Lee HK;Ketteler R;Kriston-Vizi J;Cutler DF
细胞器大小的变化往往与其功能的改变有关。内皮细胞储存von Willebrand因子(VWF)，这是一种在韦贝尔-帕拉德小体(WPB)中对止血至关重要的糖蛋白，是一种雪茄形状的分泌颗粒，产生的大小不一。我们最近发现，强迫改变WPB的大小会改变这种货物的活性。我们现在发现，用他汀类药物处理的内皮细胞产生较短的WPBs，并且它们在胞吐时释放的vWF显示出将血小板招募到内皮细胞表面的能力降低。我们还研究了白细胞大小改变的其他功能后果，我们还报告了在胞吐时形成的内皮表面相关的vWF招募可溶性血浆vWF，这一过程可以通过缩短白细胞的处理而减少，包括他汀类药物。这些结果表明，vWF对血小板和血管内皮细胞表面血浆vWF的胞吐后黏附活性反映了其储存细胞器的大小。因此，我们的发现表明，WPB大小的变化通过影响其vWF货物的黏附活性，可能代表了一种新的调节血管壁上血小板聚集的模式。
Changes in the size of cellular organelles are often linked to modifications in their function. Endothelial cells store von Willebrand Factor (vWF), a glycoprotein essential to haemostasis in Weibel-Palade bodies (WPBs), cigar-shaped secretory granules that are generated in a wide range of sizes. We recently showed that forcing changes in the size of WPBs modifies the activity of this cargo. We now find that endothelial cells treated with statins produce shorter WPBs and that the vWF they release at exocytosis displays a reduced capability to recruit platelets to the endothelial cell surface. Investigating other functional consequences of size changes of WPBs, we also report that the endothelial surface-associated vWF formed at exocytosis recruits soluble plasma vWF and that this process is reduced by treatments that shorten WPBs, statins included. These results indicate that the post-exocytic adhesive activity of vWF towards platelets and plasma vWF at the endothelial surface reflects the size of their storage organelle. Our findings therefore show that changes in WPB size, by influencing the adhesive activity of its vWF cargo, may represent a novel mode of regulation of platelet aggregation at the vascular wall.
影响因子：
4.6
作者：
Ferraro F;Mafalda Lopes da S;Grimes W;Lee HK;Ketteler R;Kriston-Vizi J;Cutler DF
通讯作者：
Cutler DF