Noninvasive diagnosis of intraamniotic infection: proteomic biomarkers in vaginal fluid
Noninvasive diagnosis of intraamniotic infection: proteomic biomarkers in vaginal fluid
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DOI：
10.1016/j.ajog.2010.03.037
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发表时间：
2010-07-01
影响因子：
9.8
通讯作者：
Nagalla, Srinivasa R.
中科院分区：
文献类型：
--
作者：
Hitti, Jane;Lapidus, Jodi A.;Nagalla, Srinivasa R.
目的：我们分析了阴道液蛋白质组，以确定生物标志物羊膜内感染的妇女在早产labor.Study设计：蛋白质组分析进行阴道液标本从早产的妇女，使用多维液相色谱，串联质谱，和无标记的定量。酶免疫测定用于定量候选蛋白质。羊膜内感染（阳性羊水细菌培养和/或白细胞介素-6> 2 ng/mL）的分类准确性进行了评估，使用receiver-operator特征曲线通过logistic regression.RESULTS获得：在170例受试者中，30例（18%）有羊膜内感染。阴道液蛋白质组分析显示338个独特的蛋白质。无标记定量鉴定了15种在羊膜内感染中差异表达的蛋白质，包括急性期反应物、免疫调节剂、高丰度羊水蛋白和细胞外基质信号传导因子;这些发现通过酶免疫测定法得到证实。一个多分析物算法显示准确的分类intraamnioinfection.CONCLUSION：阴道液蛋白质组分析确定的蛋白质能够区分患者与非intraamnioinfection。
OBJECTIVE: We analyzed the vaginal fluid proteome to identify biomarkers of intraamniotic infection among women in preterm labor.STUDY DESIGN: Proteome analysis was performed on vaginal fluid specimens from women with preterm labor, using multidimensional liquid chromatography, tandem mass spectrometry, and label-free quantification. Enzyme immunoassays were used to quantify candidate proteins. Classification accuracy for intraamniotic infection (positive amniotic fluid bacterial culture and/or interleukin-6 > 2 ng/mL) was evaluated using receiver-operator characteristic curves obtained by logistic regression.RESULTS: Of 170 subjects, 30 (18%) had intraamniotic infection. Vaginal fluid proteome analysis revealed 338 unique proteins. Label-free quantification identified 15 proteins differentially expressed in intraamniotic infection, including acute-phase reactants, immune modulators, high-abundance amniotic fluid proteins and extracellular matrix-signaling factors; these findings were confirmed by enzyme immunoassay. A multi-analyte algorithm showed accurate classification of intraamniotic infection.CONCLUSION: Vaginal fluid proteome analyses identified proteins capable of discriminating between patients with and without intraamniotic infection.