In vivo analysis of Purkinje cell firing properties during postnatal mouse development
In vivo analysis of Purkinje cell firing properties during postnatal mouse development
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DOI：
10.1152/jn.00586.2014
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发表时间：
2015-01-15
影响因子：
2.5
通讯作者：
Sillitoe, Roy V.
中科院分区：
文献类型：
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作者：
Arancillo, Marife;White, Joshua J.;Sillitoe, Roy V.
浦肯野细胞活动对于控制运动行为至关重要。在运动行为期间，浦肯野细胞激发两种类型的动作电位：本质上产生的简单尖峰和由攀爬纤维输入引起的复杂尖峰。尽管这些尖峰的功能越来越清楚，但它们是如何建立的仍然知之甚少。在这里，我们使用在麻醉和清醒小鼠中进行的体内电生理学方法来记录从出生后第二周发育到成年的浦肯野细胞活性。我们发现，复杂的尖峰放电率在 3 周龄时急剧增加，而简单的尖峰放电率直到 4 周龄才逐渐增加。我们还发现，与成体相比，发育过程中简单的尖峰放电模式更加不规则，因为细胞倾向于爆发性放电，并被长时间的停顿打断。简单穗放电的规律性仅在 4 周龄时达到成熟。相比之下，成人复杂的尖峰模式在生命的第二周就已经很明显，并且在所有年龄段都保持一致。对行为警觉的小鼠的浦肯野细胞的分析表明，在完成关键的形态发生过程（例如迁移、层压和叶化）一周多后，才达到成年模式。因此，浦肯野细胞活性在整个出生后发育过程中动态变化，经历了电路形成所需的几个关键事件。总的来说，我们表明简单的尖峰和复杂的尖峰放电具有独特的发育轨迹。
Purkinje cell activity is essential for controlling motor behavior. During motor behavior Purkinje cells fire two types of action potentials: simple spikes that are generated intrinsically and complex spikes that are induced by climbing fiber inputs. Although the functions of these spikes are becoming clear, how they are established is still poorly understood. Here, we used in vivo electrophysiology approaches conducted in anesthetized and awake mice to record Purkinje cell activity starting from the second postnatal week of development through to adulthood. We found that the rate of complex spike firing increases sharply at 3 wk of age whereas the rate of simple spike firing gradually increases until 4 wk of age. We also found that compared with adult, the pattern of simple spike firing during development is more irregular as the cells tend to fire in bursts that are interrupted by long pauses. The regularity in simple spike firing only reached maturity at 4 wk of age. In contrast, the adult complex spike pattern was already evident by the second week of life, remaining consistent across all ages. Analyses of Purkinje cells in alert behaving mice suggested that the adult patterns are attained more than a week after the completion of key morphogenetic processes such as migration, lamination, and foliation. Purkinje cell activity is therefore dynamically sculpted throughout postnatal development, traversing several critical events that are required for circuit formation. Overall, we show that simple spike and complex spike firing develop with unique developmental trajectories.