Brush border motility. Microvillar contraction in triton-treated brush borders isolated from intestinal epithelium.
Brush border motility. Microvillar contraction in triton-treated brush borders isolated from intestinal epithelium.
复制标题
刷边界运动。从肠上皮分离的特里顿处理的刷子边界中的微绒毛收缩。
DOI：
10.1083/jcb.71.2.417
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发表时间：
1976-11
影响因子：
7.8
通讯作者：
Mooseker, M S
中科院分区：
文献类型：
--
作者：
Mooseker, M S
肠上皮细胞的刷状边缘由一系列紧密排列的微绒毛组成。每个微绒毛内有一束20-30个肌动蛋白丝。细丝束的基端嵌入并通过丝状网（终端网）相互连接，终端网位于微绒毛的正下方。当钙和ATP被添加到用清洁剂Triton X-100处理过的分离的刷状边缘时，微绒毛细丝束迅速缩回并通过终端网区。刷状边界收缩蛋白的生化研究表明，微绒毛收缩是由肌动球蛋白介导的。我们以前已经表明，刷缘肌动蛋白的主要蛋白质（Tilney, l.g., and m.s. Mooseker. 1971）。Proc。国家的。学会科学。（美国法典68:2611-2615）。刷状边缘还含有一种与肌球蛋白重链亚基（200,000道尔顿）分子量相同的蛋白质。此外，脱膜刷状边缘的制备显示钾- edta atp酶活性为0.02 μ mol磷酸/mg-min（22℃）；该试验可诊断从脊椎动物中分离的肌球蛋白样atp酶。刷状边缘的其他蛋白质包括与原肌球蛋白性质相似的30,000道尔顿蛋白质，以及与Z带蛋白具有相同分子量的α -肌动蛋白（95,000道尔顿）。这些观察结果如何以体内微绒毛运动为基础，在我们最近的刷状边界肌动蛋白和肌球蛋白组织模型的框架内进行了讨论（Mooseker， M. S.和L. G. Tilney. 1975）。[j] .细胞生物学杂志。67:725-743。
The brush border of intestinal epithelial cells consists of an array of tightly packed microvilli. Within each microvillus is a bundle of 20-30 actin filaments. The basal ends of the filament bundles are embedded in and interconected by a filamentous meshwork, the terminal web, which lies directly beneath the microvilli. When calcium and ATP are added to isolated brush borders that have been treated with the detergent, Triton X-100, the microvillar filament bundles rapidly retract into and through the terminal web region. Biochemical studies of brush border contractile proteins suggest that the observed microvillar contraction is actomyosin mediated. We have shown previously that the major protein of the brush border's actin (Tilney, L. G., and M. S. Mooseker. 1971. Proc. Natl. Acad. Sci. U. S. A. 68:2611-2615). The brush border also contains a protein with the same molecular weight as the heavy chain subunit of myosin (200, 000 daltons). In addition, preparations of demembranated brush borders exhibit potassium-EDTA ATPase activity of 0.02 mumol phosphate/mg-min (22 degrees C); this assay is diagnostic for myosin-like ATPase isolated from vertebrate sources. Other proteins of the brush border include a 30,000 dalton protein with properties similar to those of tropomyosin, and a protein with the same molecular weight as the Z band protein, alpha-actinin (95,000 daltons). How these observations bear on the basis for microvillar movements in vivo is discussed within the framework of our recent model for the organization of actin and myosin in the brush border (Mooseker, M. S., and L. G. Tilney. 1975. J. Cell Biol. 67:725-743).