Co-culturing mesenchymal stem cells from bone marrow and periosteum enhances osteogenesis and neovascularization of tissue-engineered bone
Co-culturing mesenchymal stem cells from bone marrow and periosteum enhances osteogenesis and neovascularization of tissue-engineered bone
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共培养骨髓和骨膜间充质干细胞增强组织工程骨的成骨和新生血管形成
DOI：
10.1002/term.489
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发表时间：
2012-11-01
影响因子：
3.3
通讯作者：
Zeng, Bingfang
中科院分区：
文献类型：
--
作者：
Chen, Daoyun;Zhang, Xianlong;Zeng, Bingfang
骨髓间充质干细胞（Mesenchymal stem cells，MSCs）是骨组织工程的重要细胞来源。本研究表明，共培养的人骨髓干细胞（hBMSCs）和骨膜来源的干细胞（hPCs）导致在体外和体内的成骨分化的协同效应。与hBMSCs和hPCs相比，共培养的MSCs在体外表现出丰富的矿化、稳定的钙沉积、稳定增加的ALP活性以及成骨特异性基因（COL 1A 1、BMP-2、骨桥蛋白、骨钙素）mRNA表达的增强。在免疫缺陷小鼠中植入细胞β-TCP支架8周后，在体内评价总新骨形成、成熟骨形成和新血管形成期间证实了类似的协同效应。基于这些发现，使用共培养的hBMSCs和hPCs可以推荐作为一个有前途的新方法，骨组织工程的应用。版权所有（c）2011约翰威利父子有限公司
Mesenchymal stem cells (MSCs) isolated from bone marrow and periosteum are often used as cellular sources for bone tissue engineering. This study showed that co-cultured human bone marrow stem cells (hBMSCs) and periosteal-derived stem cells (hPCs) resulted in a synergistic effect on osteogenic differentiation both in vitro and in vivo. Compared to hBMSCs and hPCs, co-culturing MSCs showed abundant mineralization, robust calcium deposition, steadily increasing ALP activity, and upgraded mRNA expression of osteogenic specific genes (COL1A1, BMP-2, osteopontin, osteocalcin) in vitro. Eight weeks after implantation of cellular beta-TCP scaffolds in immunodeficient mice, similar synergistic effects were confirmed during in vivo evaluation of total new bone formation, mature bone formation, and neovascularization. Based on these findings, the use of co-cultured hBMSCs and hPCs can be recommended as a promising new approach for bone tissue engineering applications. Copyright (c) 2011 John Wiley & Sons, Ltd.