Printable Organic Electronic Materials for Precisely Positioned Cell Attachment
Printable Organic Electronic Materials for Precisely Positioned Cell Attachment
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用于精确定位电池附着的可印刷有机电子材料
DOI：
10.1021/acs.langmuir.0c03319
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发表时间：
2021
期刊：
影响因子：
3.9
通讯作者：
Forrest, Stephen R.
中科院分区：
文献类型：
--
作者：
Horowitz, Jeffrey A.;Zhong, Xiaoyang;DePalma, Samuel J.;Ward Rashidi, Maria R.;Baker, Brendon M.;Lahann, Joerg;Forrest, Stephen R.
在过去的30年里，组织工程学和有机电子学的研究都有了巨大的扩展。尽管这两个领域的互动很少，但随着有机电子学的兴起，材料和制造技术提供了创新和转化的潜力，如果适当地适应于组织工程的生物材料模式。在这项工作中，我们使用两种有机电子材料作为生物相容性聚对二甲苯表面的粘附点。有机电子材料是通过真空热蒸发和有机蒸汽喷射打印精确沉积的，这是制造有机电子设备所使用的经过验证的、可扩展的工艺。小分子量有机物可防止聚乙二醇甲基丙烯酸酯防污聚合物刷在分子贴片之间的空隙中生长，从而使这些背景区域具有蛋白质和细胞抗性。最后，纤维连接蛋白附着在分子贴片上，允许成纤维细胞选择性黏附。该工艺简单、可重复，并促进了细胞在目标位置的高产率附着，表明生物兼容的有机小分子材料可以利用电子设备制造中广泛使用的技术在微尺度上对细胞进行图案设计。
Over the past 3 decades, there has been a vast expansion of research in both tissue engineering and organic electronics. Although the two fields have interacted little, the materials and fabrication technologies which have accompanied the rise of organic electronics offer the potential for innovation and translation if appropriately adapted to pattern biological materials for tissue engineering. In this work, we use two organic electronic materials as adhesion points on a biocompatible poly(p-xylylene) surface. The organic electronic materials are precisely depositedviavacuum thermal evaporation and organic vapor jet printing, the proven, scalable processes used in the manufacture of organic electronic devices. The small molecular-weight organics prevent the subsequent growth of antifouling polyethylene glycol methacrylate polymer brushes that grow within the interstices between the molecular patches, rendering these background areas both protein and cell resistant. Last, fibronectin attaches to the molecular patches, allowing for the selective adhesion of fibroblasts. The process is simple, reproducible, and promotes a high yield of cell attachment to the targeted sites, demonstrating that biocompatible organic small-molecule materials can pattern cells at the microscale, utilizing techniques widely used in electronic device fabrication.