High-Throughput Sequencing of miRNAs Reveals a Tissue Signature in Gastric Cancer and Suggests Novel Potential Biomarkers.
High-Throughput Sequencing of miRNAs Reveals a Tissue Signature in Gastric Cancer and Suggests Novel Potential Biomarkers.
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DOI：
10.4137/bbi.s23773
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发表时间：
2015
影响因子：
5.8
通讯作者：
Ribeiro-Dos-Santos Â
中科院分区：
文献类型：
--
作者：
Darnet S;Moreira FC;Hamoy IG;Burbano R;Khayat A;Cruz A;Magalhães L;Silva A;Santos S;Demachki S;Assumpção M;Assumpção P;Ribeiro-Dos-Santos Â
胃癌在全球范围内发病率和死亡率均较高；然而，生物标志物在临床诊断中的应用仍然有限。 microRNA (miRNA) 是具有识别风险和预后以及治疗靶点潜力的生物标志物。我们在没有肿瘤样本的情况下对胃腺癌和胃窦进行了超深 miRnomes 测序。我们观察到，一小部分样本负责大约 80% 的总 miRNA 表达，这可能代表了 miRNA 组织特征。此外，我们还发现了 7 个表现出显着差异的 miRNA，其中 hsa-miR-135b 和 hsa-miR-29c 能够区分无肿瘤胃窦和胃癌，无论组织学类型如何。这些发现通过定量实时聚合酶链反应得到了验证。结果显示，hsa-miR-135b 和 hsa-miR-29c 是潜在的胃腺癌发生生物标志物，能够识别罹患这种癌症的风险较高的个体，甚至可以用作治疗靶点，实现个体化临床管理。
Gastric cancer has a high incidence and mortality rate worldwide; however, the use of biomarkers for its clinical diagnosis remains limited. The microRNAs (miRNAs) are biomarkers with the potential to identify the risk and prognosis as well as therapeutic targets. We performed the ultradeep miRnomes sequencing of gastric adenocarcinoma and gastric antrum without tumor samples. We observed that a small set of those samples were responsible for approximately 80% of the total miRNAs expression, which might represent a miRNA tissue signature. Additionally, we identified seven miRNAs exhibiting significant differences, and, of these, hsa-miR-135b and hsa-miR-29c were able to discriminate antrum without tumor from gastric cancer regardless of the histological type. These findings were validated by quantitative real-time polymerase chain reaction. The results revealed that hsa-miR-135b and hsa-miR-29c are potential gastric adenocarcinoma occurrence biomarkers with the ability to identify individuals at a higher risk of developing this cancer, and could even be used as therapeutic targets to allow individualized clinical management.