Prebiotic carbohydrates modify the mucosa associated microflora of the human large bowel
Prebiotic carbohydrates modify the mucosa associated microflora of the human large bowel
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DOI：
10.1136/gut.2003.037580
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发表时间：
2004-11-01
期刊：
影响因子：
24.5
通讯作者：
Cummings, JH
中科院分区：
文献类型：
--
作者：
Langlands, SJ;Hopkins, MJ;Cummings, JH
背景和目标：大肠粘膜相关的植物群在决定粘膜功能方面是重要的，尽管控制其组成的因素尚不清楚。本研究确定了益生元碳水化合物低聚果糖和菊粉对粘膜植物群的影响。方法：使用了一种体外恒化器模型，该模型既包含了益生菌，也包含了表面相关细菌，随后对29名接受结肠镜检查的受试者进行了干预研究。从结肠镜检查等待名单中招募的14名受试者，用7.5 g低聚果糖和7.5 g菊粉的混合物补充他们的饮食两周。在结肠镜检查时招募了15名受试者，未给予补充剂。从盲肠、横结肠、降结肠和直肠进行多处内镜活检。粘膜植物群的特征在于文化和种属水平的细胞脂肪酸谱。结果：体外益生元使细菌表面计数从6.6增加到7.3 log（10）菌落形成单位（CFU）/载玻片（p< 0.0006），而细菌表面计数没有显著变化。在喂养研究中，益生元增加了近端（对照5.3（0.4）v益生元6.3（0.3））（p = 0.059）和远端（对照5.2（0.3）v益生元6.4（0.3））结肠（p = 0.01）中的粘膜生物多样性细菌（log CFU/g粘膜（SEM））。乳酸杆菌也增加（近端结肠中分别为3.0（0.1）v3.7（0.2）（p = 0.02），远端结肠中分别为3.1（0.1）v3.6（0.2）（p = 0.04））。有显着更多的真细菌在喂养的科目，但总厌氧菌梭菌，类杆菌，或大肠菌群没有变化，也没有在增殖indics.Conclusion：益生元碳水化合物可以改变粘膜相关的植物群的组成显着。
Background and aims: The mucosa associated flora of the large intestine is important in determining mucosal function although what controls its composition is unknown. This study has determined the effect of the prebiotic carbohydrates oligofructose and inulin on the mucosal flora.Methods: An in vitro chemostat model of both planktonic and surface associated bacteria was used followed by an intervention study in 29 subjects undergoing colonoscopy.Subjects: Fourteen subjects, recruited from colonoscopy waiting lists, supplemented their diet for two weeks with a mix of 7.5 g of oligofructose and 7.5 g inulin. Fifteen subjects were recruited at the time of colonoscopy and given no supplement. Multiple endoscopic biopsies were taken from the caecum, transverse and descending colon, and rectum. The mucosal flora was characterised by culture and to species level by cellular fatty acid profiles. Cell proliferation was assessed by immunohistochemical staining for minichromosome maintenance protein 2, Ki67, and proliferating cell nuclear antigen.Results: In vitro prebiotics increased surface counts of bifidobacteria from 6.6 to 7.3 log(10) colony forming units (CFU) per slide ( p< 0.0006) with no significant changes in planktonic bacteria. In the feeding study, prebiotics increased mucosal bifidobacteria ( log CFU/g mucosa (SEM)) in both the proximal ( control 5.3 (0.4) v prebiotic 6.3 (0.3)) ( p = 0.059) and distal ( control 5.2 ( 0.3) v prebiotic 6.4 ( 0.3)) colon ( p = 0.01). Lactobacilli were also increased (3.0 (0.1) v 3.7 (0.2) ( p = 0.02) in the proximal and 3.1 ( 0.1) v 3.6 ( 0.2) ( p = 0.04) in the distal colon, respectively). There were significantly more eubacteria in fed subjects but no changes in total anaerobes clostridia, bacteroides, or coliforms, nor in proliferation indices.Conclusion: Prebiotic carbohydrates can change the composition of the mucosa associated flora significantly.