Amount of daily exercise is an essential stimulation to alter the epigenome of skeletal muscle in rats.
Amount of daily exercise is an essential stimulation to alter the epigenome of skeletal muscle in rats.
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每日运动量是改变大鼠骨骼肌表观基因组的重要刺激。
DOI：
10.1152/japplphysiol.00074.2018
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发表时间：
2018
影响因子：
3.3
通讯作者：
and Kawano F
中科院分区：
文献类型：
--
作者：
Ohsawa I;Konno R;Masuzawa R;and Kawano F
长期跑步训练会导致骨骼肌的表观遗传变化。在这里，我们测试了跑步训练总量或持续时间对大鼠足底肌组蛋白分布的影响。将断奶后的幼鼠分为3个训练组：Run-1组，每天30min，在24m/min的跑台上跑8wk；Run-2，15min/d，8wk；尽管在Run-3中观察到柠檬酸合成酶活性略有增加，但跑步训练对柠檬酸合成酶活性没有显著影响。以前被定义为对跑步训练表现出典型反应的基因被定位为使用染色质免疫沉淀来测量组蛋白的分布。在Run-2和Run-3中，乙酰化组蛋白3的分布增加，但在Run-1中没有。H3.3掺入核小体在RUN-1中被刺激，而H3.3的分布在RUN-2中没有变化或在RUN-3中下调。在Run-3中也检测到H3.3表达的显著下调。我们进一步检查了目标基因在急性跑步过程中的反应。在急性跑步时，靶基因被转录激活，组蛋白乙酰化被激动组蛋白乙酰化。这些结果表明，跑步训练刺激了组蛋白成分向H3.3的交换，抑制了Run-1中乙酰化组蛋白的积累。此外，研究进一步表明，日常运动量的增加引起H3.3表达的变化，影响RUN-3的组蛋白交换速率。骨骼肌中的NEW&NOTEWORTHY染色质重塑是防止通过长期运动训练获得的晚年废用萎缩的有效机制。在这里，我们在大鼠身上证明了日常运动量是骨骼肌表观遗传变化发展的关键因素。为了获得健康益处，我们的研究表明，考虑日常运动的时间耐力是重要的。
Long-term running training causes epigenetic changes in the skeletal muscles. Here we tested the effects of the total amount or duration of running training on the distribution of histones in the rat plantaris muscle. Post-weaned young rats were assigned to 3 different training groups: Run-1, 30 min/day running exercise for 8 wk using an animal treadmill at 24 m/min; Run-2, 15 min/day for 8 wk; and Run-3, 60 min/day for 4 wk. Citrate synthase activity was not significantly changed by running training, although the slight increase was observed in Run-3. Genes that were previously defined as showing the typical responses to running training were targeted to measure the distribution of histones using chromatin immunoprecipitation. The distribution of acetylated histone 3 was elevated in Run-2 and Run-3, but not in Run-1. Incorporation of H3.3 into the nucleosome was stimulated in Run-1, whereas H3.3 distribution was unchanged in Run-2 or downregulated in Run-3. Significant downregulation of H3.3 expression was also detected in Run-3. We further checked the responses of the target genes during acute running. Target genes were transcriptionally activated and histone acetylation was stimulated at the loci in response to acute running. These results suggested that the exchange of the histone component to H3.3 was stimulated by running training, inhibiting the accumulation of acetylated histones in Run-1. Additionally, it was further suggested that the enhanced daily amount of running caused changes in the H3.3 expression, affecting the rate of the histone exchange in Run-3.NEW & NOTEWORTHYChromatin remodeling in the skeletal muscle is a potent mechanism preventing disuse atrophy in later life that can be acquired via long-term exercise training. Here we demonstrated in rats that daily exercise amount is a key factor in the development of epigenetic changes in the skeletal muscle. To acquire a health benefit, our research suggests the importance of considering the time endurance for daily exercise bouts.