Dissection of Two Acyl-Transfer Reactions Centered on Acyl-S-Carrier Protein Intermediates for Incorporating 5-Chloro-6-methyl-O-methylsalicyclic Acid into Chlorothricin
Dissection of Two Acyl-Transfer Reactions Centered on Acyl-S-Carrier Protein Intermediates for Incorporating 5-Chloro-6-methyl-O-methylsalicyclic Acid into Chlorothricin
复制标题
解析以酰基-S-载体蛋白中间体为中心的两个酰基转移反应，用于将 5-氯-6-甲基-O-甲基水杨酸掺入氯丝菌素中
DOI：
10.1002/cbic.200800714
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发表时间：
2009-03-23
期刊：
影响因子：
3.2
通讯作者：
Liu, Wen
中科院分区：
文献类型：
--
作者：
He, Qing-Li;Jia, Xin-Ying;Liu, Wen
使微生物为我们工作：在氯丝菌素的生物合成途径中阐明了一种以酰基载体蛋白为中心的策略，该策略涉及两个不同的酰基转移步骤，用于生成和区域特异性连接5-氯-6-甲基-O-甲基水杨基到D-橄榄糖的C3′位置。酰基转移酶相关中间体突变体的鉴定验证了高度保守的Cys残基的关键作用，其通过在两步过程中使用其硫醇盐侧链作为亲核试剂来引导酰基转移。
Making the microbes work for us: An acyl‐carrier‐protein‐centered strategy that involves two distinct acyl‐transfer steps for generation and regiospecific attachment of the 5‐chloro‐6‐methyl‐O‐methylsalicyl group onto the C3′ position ofD‐olivose was elucidated in the biosynthetic pathway of chlorothricin. Identification of the mutant of the acyltransferase‐associated intermediate validated the critical role of the highly conserved Cys residue, which channels the acyl transfer by using its thiolate side chain as a nucleophile in a two‐step process.