ADVERSE-EFFECTS OF STRENUOUS EXERCISE - A DENSITOMETRIC AND HISTOMORPHOMETRIC STUDY IN THE RAT
ADVERSE-EFFECTS OF STRENUOUS EXERCISE - A DENSITOMETRIC AND HISTOMORPHOMETRIC STUDY IN THE RAT
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DOI：
10.1152/jappl.1994.76.5.1999
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发表时间：
1994-05-01
影响因子：
3.3
通讯作者：
ALEXANDRE, C
中科院分区：
文献类型：
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作者：
BOURRIN, S;GENTY, C;ALEXANDRE, C
为了研究不同骨骼部位和同一骨骼部位内松质骨适应剧烈训练的方式，对5周龄雄性大鼠进行了11周的强化跑台跑步[最大O-2消耗量（Vo（2 max））的80%]。测量Vo（2 max）、胫骨长度和骨密度。在对侧胫骨近端的骨骺、原发性松质骨（1度sp）和继发性松质骨（2度sp）以及胸椎和腰椎的2度sp中进行组织形态计量学分析。Vo（2 max）增加39%。在椎骨中未观察到变化。胫骨长度、1度sp骨体积和骨小梁数量显著减少，表明纵向骨生长迟缓。胫骨近端骨密度显著降低。在骨骺，骨小梁变薄，骨小梁数量增加。在2度sp时，骨体积和骨小梁数量显著减少。总侵蚀表面的增加可能表明骨吸收活性的早期但短暂的增加。类骨质厚度减少，而破骨细胞数量和类骨质表面不变，表明观察到的骨丢失主要是由于成骨细胞活性受损。结论：1）大强度训练降低了幼鼠纵向骨生长并诱导骨丢失; 2）松质骨适应具有部位特异性; 3）骨丢失主要是由于成骨细胞活性降低而不是骨重建的整体适应。
To investigate the manner in which cancellous bone in different skeletal sites and within a bone site adapts to strenuous training, 5-wk-old male rats were subjected to intensive treadmill running [80% of maximal O-2 consumption (Vo(2 max))] for 11 wk. Vo(2 max), tibia length, and bone mineral density were measured. Histomorphometric analysis was performed in the epiphysis, primary spongiosa (1 degrees sp) and secondary spongiosa (2 degrees sp) of the contralateral proximal tibia, and the 2 degrees sp of thoracic and lumbar vertebrae. Vo(2 max) was increased by 39%. No changes were observed in vertebrae. Tibia length, 1 degrees sp bone volume, and number of trabeculae were significantly decreased, indicating a retarded longitudinal bone growth. Bone mineral density in the proximal tibia was significantly decreased. In the epiphysis, a trabecular thinning and an increase of trabecular number were shown. In the 2 degrees sp, bone volume and number of trabeculae were significantly decreased. The increased total eroded surfaces could indicate an early but transient increase in bone resorption activity. Osteoid thickness was reduced, whereas osteoclast number and osteoid surfaces were unchanged, suggesting that the observed bone loss was mostly due to an impaired osteoblastic activity. In conclusion, 1) strenous training in young rats reduces longitudinal bone growth and induces bone loss, 2) the cancellous bone adaptation is site specific, and 3) the bone loss is mainly due to decreased osteoblastic activity rather than a global adaptation of bone remodeling.