Preparation and characterization of monoclonal antibodies recognizing three distinct differentiation antigens (BL1, BL2, BL3) on human B lymphocytes.
Preparation and characterization of monoclonal antibodies recognizing three distinct differentiation antigens (BL1, BL2, BL3) on human B lymphocytes.
复制标题
识别人 B 淋巴细胞上三种不同分化抗原（BL1、BL2、BL3）的单克隆抗体的制备和表征。
作者：
C. Y. Wang;W. Azzo;A. Al;N. Chiorazzi;D. Knowles
在本文中，我们报告了三种单克隆抗体，命名为α BL 1，α BL 2，和α BL 3，识别独特的抗原无关的补体，Fc和小鼠红细胞花环受体，这是优先表达的B淋巴细胞的生成和表征。α BL 1识别热稳定的非免疫沉淀抗原，可能是天然的糖脂。Alpha BL 2识别分子量为100的不可还原的单一多肽68，000个，偶尔与HLA-DR抗原的p29，34复合物共沉淀。α BL 3识别具有m.w. 105，000的酸性pI点。我们证明，BL 1表达在胎肝造血细胞，一个小的子集（5至15%）的Ficoll-Hypaque分离的正常骨髓细胞，和非粘附，非E玫瑰花结形成细胞和粒细胞的亚群。BL 2表达于胎肝造血细胞、3 - 7%的正常骨髓细胞和大部分（40 - 70%）非贴壁、非E玫瑰花结形成细胞上，具有与HLA-DR相似的独特模式。BL 3表达于非贴壁、非E玫瑰花结形成细胞的亚群和单核细胞富集的贴壁细胞群中的偶见细胞上。BL 2的峰值荧光显著高于BL 1和BL 3的峰值荧光，表明BL 2抗原密度较高。所有三种抗原均不存在于从各种淋巴组织获得的胸腺细胞和E玫瑰花结阳性T细胞组分中。BL抗原在各种特征明确的造血细胞系、白血病和恶性淋巴瘤上的细胞分布，以及体外活化和TPA诱导实验的结果表明，BL 1在B细胞分化的早期发育阶段表达，而BL 3在后期表达。BL 2表达跨越B细胞分化的未成熟和成熟阶段，成熟浆细胞除外。这里描述的α BL抗体应证明是有用的B细胞分化的调查和淋巴肿瘤的临床诊断。
We report in this paper the generation and characterization of three monoclonal antibodies, designated alpha BL1, alpha BL2, and alpha BL3, that recognize distinctive antigens unrelated to complement, Fc, and mouse erythrocyte rosette receptors, which are preferentially expressed on B lymphocytes. alpha BL1 recognizes a heat stable nonimmunoprecipitable antigen, possibly glycolipid in nature. Alpha BL2 recognizes a nonreducible single polypeptide with a m.w. of 68,000 that occasionally co-precipitates with a p29,34 complex of HLA-DR antigens. Alpha BL3 recognizes a nonreducible single polypeptide with a m.w. of 105,000 with an acidic pI point. We demonstrated that BL1 is expressed on fetal liver hematopoietic cells, a small subset (5 to 15%) of Ficoll-Hypaque-separated normal bone marrow cells, and on a subpopulation of nonadherent, non-E rosette-forming cells and granulocytes. BL2 is expressed on fetal liver hematopoietic cells, on 3 to 7% of normal bone marrow cells, and on a majority (40 to 70%) of nonadherent, non-E rosette-forming cells with a distinctive pattern similar to that of HLA-DR. BL3 is expressed on a subpopulation of nonadherent, non-E rosette-forming cells, and on occasional cells in the monocyte-enriched adherent cell population. The peak fluorescence for BL2 is substantially higher than that of BL1 and BL3, indicating higher BL2 antigen density. All three antigens are absent from thymocytes and E rosette-positive T cell fractions obtained from various lymphoid tissues. Cellular distribution of the BL antigens on various well-characterized established hematopoietic cell lines, leukemias, and malignant lymphomas, in conjunction with the results of the in vitro activation and TPA-induction experiments, suggest that BL1 is expressed during early developmental stages of B cell differentiation, whereas BL3 is expressed at the later stages. BL2 expression spans immature and mature stages of B cell differentiation, with the exception of mature plasma cells. The alpha BL antibodies described here should prove to be useful in the investigation of B cell differentiation and in the clinical diagnosis of lymphoid neoplasms.