Polarized Distribution of Glycine Transporter Isoforms in Epithelial and Neuronal Cells
Polarized Distribution of Glycine Transporter Isoforms in Epithelial and Neuronal Cells
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上皮细胞和神经元细胞中甘氨酸转运蛋白亚型的极化分布
DOI：
10.1006/mcne.1999.0807
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发表时间：
2000
影响因子：
3.5
通讯作者：
F. Zafra
中科院分区：
文献类型：
--
作者：
Irene Poyatos;F. Ruberti;R. Martı́nez;C. Giménez;C. Dotti;F. Zafra
依赖Na(+)和Cl(-)的神经递质转运体在极化细胞表面的不对称分布似乎是该基因家族的普遍特征。在本研究中，我们分析了甘氨酸转运蛋白GLYT1和GLYT2的不同亚型在极化的MDCK细胞和海马神经元中异源表达后的亚细胞分布。我们的结果表明，甘氨酸转运蛋白是以一种不对称的形式和细胞类型特异性的方式分布的。GLYT1b定位于MDCK细胞和神经元的基底侧域和体树突域。然而，GLYT1a在神经元中是体树突状的，但主要在MDCK细胞的顶面表达。GLYT2的两种异构体(GLYT2a和GLYT2b)存在于上皮细胞的顶面，但在神经元中分布均匀。通过定点突变，我们已经能够在GLYT1的氨基末端区域和位于该蛋白羧基尾部的两个二亮氨酸基序中识别出碱侧/躯体树突定位的信号。这些结果有助于确定极化细胞表面转运蛋白不对称分布的机制。
Asymmetrical distribution of Na(+)- and Cl(-)-dependent neurotransmitter transporters on the cell surface of polarized cells seems to be a generalized feature in this gene family. In the present study we analyzed the subcellular distribution of the various isoforms of the glycine transporters GLYT1 and GLYT2 after heterologous expression in polarized MDCK cells and in hippocampal neurons. Our results indicate that glycine transporters are asymmetrically distributed in an isoform- and cell-type-specific manner. GLYT1b is localized in the basolateral and somatodendritic domains of MDCK cells and neurons, respectively. However, GLYT1a is somatodendritic in neurons but is predominantly expressed in the apical surface of MDCK cells. The two isoforms of GLYT2 (GLYT2a and GLYT2b) are found at the apical surface in epithelial cells but are uniformly distributed in neurons. By using site-directed mutagenesis we have been able to identify signals for basolateral/somatodendritic localization in the amino-terminal region of GLYT1 and in two dileucine motifs located in the carboxyl tail of this protein. These results contribute to defining the mechanisms of asymmetrical distribution of transporters on the cell surface of polarized cells.
DOI：
10.1073/pnas.89.15.7189
发表时间：
1992-08-01
影响因子：
11.1
作者：
GUASTELLA, J;BRECHA, N;DAVIDSON, N
通讯作者：
DAVIDSON, N
轴突 γ-氨基丁酸转运蛋白 GAT-1 被分选至极化上皮细胞的顶膜。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Pietrini,G;Suh,YJ;Edelmann,L;Rudnick,G;Caplan,MJ
通讯作者：
Caplan,MJ
影响因子：
3.6
作者：
Kyeong Man Kim;Stephen F. Kingsmore;H. Han;Teresa L. Yang-Feng;N. Godinot;Michael F. Seldin;M. G. Caron;Bruno Giros
通讯作者：
Kyeong Man Kim;Stephen F. Kingsmore;H. Han;Teresa L. Yang-Feng;N. Godinot;Michael F. Seldin;M. G. Caron;Bruno Giros