Bone marrow mesenchymal stem cells can differentiate into type II alveolar epithelial cells in vitro
Bone marrow mesenchymal stem cells can differentiate into type II alveolar epithelial cells in vitro
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DOI：
10.1042/cbi20110026
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发表时间：
2011-12-01
影响因子：
3.9
通讯作者：
Zhong, Hong
中科院分区：
文献类型：
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作者：
Ma, Nan;Gai, Hui;Zhong, Hong
在本研究中，我们证明了骨髓间充质干细胞（BMSCs）可以成功地分化为II型肺泡上皮细胞在体外模拟肺微环境。在改良的SAGM（小气道生长培养基）中将BMSC与MAC-5细胞共培养。BMSC衍生的II型肺泡上皮细胞在形态上类似于人肺上皮细胞。它们在共培养10天后开始出现，并在第15天后成为形态上占优势的。相应地，SPC（表面活性蛋白C），人II型肺泡上皮细胞的特异性功能标志物，在分化的细胞中检测到的RT-PCR（逆转录-FOR）分析后15天。免疫染色分析显示，目前分散SPC阳性细胞的分化效率为2.43- 4.21%。我们的研究进一步表明，SPC基因在分化细胞中的表达水平与BMSCs与MRC-5细胞的比例以及改良SAGM的成分有关。
In this study, we demonstrate that BMSCs (bone marrow mesenchymal stem cells) can be successfully differentiated into type II alveolar epithelial cells in vitro under mimic pulmonary microenvironment. BMSCs were co-cultured with MAC-5 cells in modified SAGM (small airway growth medium). The BMSC-derived type II alveolar epithelial cells morphologically resemble human lung epithelial cells. They began to appear after 10 days in co-culture and became morphologically dominant after day 15. Correspondingly, SPC (surfactant protein C), a specific functional marker of human type II alveolar epithelial cells, was detected in differentiated cells by RT-PCR (reverse transcription-FOR) analysis after day 15. Immunostaining analysis revealed the present of scattered SPC-positive cells with a differentiation efficiency of 2.43-4.21%. Our study further showed that the SPC gene expression level in differentiated cells was related to the ratio of BMSCs to MRC-5 cells and the components of modified SAGM.