Adipose tissue behavior is distinctly regulated by neighboring cells and fluid flow stress: a possible role of adipose tissue in peritoneal fibrosis
Adipose tissue behavior is distinctly regulated by neighboring cells and fluid flow stress: a possible role of adipose tissue in peritoneal fibrosis
复制标题
DOI：
10.1007/s10047-013-0702-8
复制
发表时间：
2013-03
影响因子：
1.3
通讯作者：
S. Aoki;Kazuma Udo;H. Morimoto;Satoshi Ikeda;T. Takezawa;K. Uchihashi;Aki Nishijima‐Matsunobu;M. Noguchi;H. Sugihara;S. Toda
中科院分区：
文献类型：
--
作者：
S. Aoki;Kazuma Udo;H. Morimoto;Satoshi Ikeda;T. Takezawa;K. Uchihashi;Aki Nishijima‐Matsunobu;M. Noguchi;H. Sugihara;S. Toda
脂肪组织，连同间皮层和微血管，是腹膜系膜的主要组成部分，而肠系膜是腹膜纤维化的靶点。脂肪组织已被推测在腹膜透析(PD)相关的纤维化中起作用，但脂肪组织在这一过程中的确切细胞动力学仍有待确定。为了阐明这一关键问题，我们使用了一种新的腹膜重建模型来分析脂肪组织的动力学，在该模型中可以识别间皮细胞或内皮细胞的作用。将脂肪组织与间皮细胞或内皮细胞在器官培养和流体应力培养系统中进行联合培养。取脂肪组织来源的梭形间充质细胞和未成熟脂肪细胞进行免疫组织化学染色。在该体系中培养的脂肪组织碎片在非共培养条件下产生了许多梭形间充质细胞。然而，在与间皮细胞覆盖的共培养条件下，从脂肪组织中长出的梭形间充质细胞的数量减少。间皮细胞在分离条件下共培养不能抑制脂肪组织中梭形间充质细胞的出现。有趣的是，内皮细胞在流体压力下促进了脂肪组织中富含脂质的未成熟脂肪细胞的出现。我们已经证明，在流体流动压力下，脂肪组织的行为不仅受到间皮细胞和内皮细胞的调节，还参与了腹膜纤维化和脂肪聚集的产生。我们的研究结果表明，脂肪组织是PD治疗引起腹膜纤维化的潜在细胞来源。
Adipose tissue, together with the mesothelial layer and microvessels, is a major component of the mesenteric peritoneum, and the mesenterium is a target site for peritoneal fibrosis. Adipose tissue has been speculated to play a role in peritoneal dialysis (PD)-related fibrosis, but the precise cellular kinetics of adipose tissue during this process remain to be determined. To clarify this critical issue, we analyzed the kinetics of adipose tissue using a novel peritoneal reconstruction model in which the effects of mesothelial cells or endothelial cells could be identified. Adipose tissue was co-cultured with mesothelial cells or endothelial cells in a combined organ culture and fluid flow stress culture system. Spindle mesenchymal cells and immature adipocytes derived from adipose tissue were characterized by immunohistochemistry. Adipose tissue fragments cultured in this system yielded many spindle mesenchymal cells in non-co-culture conditions. However, the number of spindle mesenchymal cells emerging from adipose tissue was reduced in co-culture conditions with a covering layer of mesothelial cells. Mesothelial cells co-cultured in the separated condition did not inhibit the emergence of spindle mesenchymal cells from adipose tissue. Interestingly, endothelial cells promoted the emergence of lipid-laden immature adipocytes from adipose tissue under fluid flow stress. We have demonstrated that adipose tissue behavior is not only regulated by mesothelial cells and endothelial cells under fluid flow stress, but is also involved in fibrosis and fat mass production in the peritoneum. Our findings suggest that adipose tissue is a potential source of cells for peritoneal fibrosis caused by PD therapy.