Global analysis of transcriptome in dorsal root ganglia following peripheral nerve injury in rats
Global analysis of transcriptome in dorsal root ganglia following peripheral nerve injury in rats
复制标题
大鼠周围神经损伤后背根神经节转录组的整体分析
DOI：
10.1016/j.bbrc.2016.07.067
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发表时间：
2016-09-09
影响因子：
3.1
通讯作者：
Yao, Chun
中科院分区：
文献类型：
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作者：
Gong, Leilei;Wu, Jiancheng;Yao, Chun
周围神经系统损伤后具有内在的再生能力，伴随着众多细胞、分子和信号通路的协调作用。这些损伤后的生物学变化是复杂的，缺乏足够的了解。因此，全面了解神经损伤后的变化，阐明神经再生的机制具有重要意义。应用RNA测序技术检测大鼠坐骨神经挤压伤后0、3、9、1、4、7 d背根神经节（DRG）神经元转录水平的变化。然后选择差异表达的基因，并根据其表达模式将其分为主要簇。对簇2（具有在9 h前高表达然后下调表达的基因）和簇6（簇4和簇5的组合，具有在1 d前低表达然后上调表达的基因）进行GO注释和KEGG途径分析。然后构建这两个簇的基因行为网络，并通过定量实时PCR验证关键基因的表达。本研究提供了有关神经损伤后DRG神经元转录组变化的有价值的信息，确定了可用于促进神经损伤后轴突再生的潜在基因，并有助于阐明周围神经损伤的生物学过程和分子机制。(C)2016 Elsevier Inc. All rights reserved.
Peripheral nervous system has intrinsic regeneration ability after injury, accompanied with the coordination of numerous cells, molecules and signaling pathways. These post-injury biological changes are complex with insufficient understanding. Thus, to obtain a global perspective of changes following nerve injury and to elucidate the mechanisms underlying nerve regeneration are of great importance. By RNA sequencing, we detected transcriptional changes in dorsal root ganglia (DRG) neurons at 0 h, 3 h, 9 h,1 d, 4 d and 7 d following sciatic nerve crush injury in rats. Differentially expressed genes were then selected and classified into major clusters according to their expression patterns. Cluster 2 (with genes high expressed before 9 h and then down expressed) and cluster 6 (combination of cluster 4 and 5 with genes low expressed before 1 d and then up expressed) were underwent GO annotation and KEGG pathway analysis. Gene act networks were then constructed for these two clusters and the expression of pivotal genes was validated by quantitative real-time PCR. This study provided valuable information regarding the transcriptome changes in DRG neurons following nerve injury, identified potential genes that could be used for improving axon regeneration after nerve injury, and facilitated to elucidate the biological process and molecular mechanisms underlying peripheral nerve injury. (C) 2016 Elsevier Inc. All rights reserved.