The mouse brain transcriptome by SAGE: Differences in gene expression between P30 brains of the partial trisomy 16 mouse model of Down syndrome (Ts65Dn) and normals
The mouse brain transcriptome by SAGE: Differences in gene expression between P30 brains of the partial trisomy 16 mouse model of Down syndrome (Ts65Dn) and normals
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DOI：
10.1101/gr.10.12.2006
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发表时间：
2000-12-01
期刊：
影响因子：
7
通讯作者：
Antonarakis, SE
中科院分区：
文献类型：
--
作者：
Chrast, R;Scott, HS;Antonarakis, SE
21三体，或唐氏综合征（DS）是智力迟钝的最常见的遗传原因。DS患者脑的神经病理学、神经化学、神经生理学和神经药理学的变化表明，中枢神经系统结构和功能的发育和维持可能异常。节段性三体性小鼠（Ts65Dn）是显示类似神经行为缺陷的DS模型。我们用基因表达系列分析（SAGE）技术研究了正常和Ts65Dn雄性和正常雌性小鼠脑（P30）的整体基因表达谱。从合并的样本中，我们总共收集了152，791个RNA标签，并在小鼠大脑转录组中观察到45，856个独特的标签。在正常男性和Ts65Dn男性大脑之间的330个统计学显著差异中，有14个核糖体蛋白基因（9个低表达），这可能意味着在DS表型的发展和维持中的异常核糖体生物合成。这项研究有助于建立一个小鼠脑转录组，并提供了第一个全面的分析基因表达的差异，在非整倍体与正常哺乳动物脑细胞。
Trisomy 21, or Down syndrome (DS) is the most common genetic cause of mental retardation. Changes in the neuropathology, neurochemistry, neurophysiology, and neuropharmacology of DS patients' brains indicate that there is probably abnormal development and maintenance of central nervous system structure and function. The segmental trisomy mouse (Ts65Dn) is a model of DS that shows analogous neurobehavioral defects. We have studied the global gene expression profiles of normal and Ts65Dn male and normal female mice brains (P30) using the serial analysis of gene expression [SAGE] technique. From the combined sample we collected a total of 152,791 RNA tags and observed 45,856 unique tags in the mouse brain transcriptome. There are 14 ribosomal protein genes (nine underexpressed) among the 330 statistically significant differences between normal male and Ts65Dn male brains, which possibly implies abnormal ribosomal biogenesis in the development and maintenance of DS phenotypes. This study contributes to the establishment of a mouse brain transcriptome and provides the first overall analysis of the differences in gene expression in aneuploid versus normal mammalian brain cells.