A survey of the bacteriophage WO in the endosymbiotic bacteria Wolbachia
A survey of the bacteriophage WO in the endosymbiotic bacteria Wolbachia
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DOI：
10.1093/molbev/msl171
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发表时间：
2007-02-01
影响因子：
10.7
通讯作者：
Vavre, Fabrice
中科院分区：
文献类型：
--
作者：
Gavotte, Laurent;Henri, Helene;Vavre, Fabrice
噬菌体是感染原核生物的常见病毒。除了它们的致命作用外，它们还参与了细菌的几个进化过程，例如编码可能对它们有益的功能蛋白，或通过转导促进水平基因转移。专性细胞内细菌的特殊生活方式通常保护它们免受噬菌体感染。然而，沃尔巴克氏体，一种细胞内的α-变形杆菌，感染不同的节肢动物和线虫物种，最有名的生殖改变，它诱导，窝藏一个噬菌体命名为WO，最近已被证明是裂解。在这里，在31个沃尔巴克氏体菌株中检查噬菌体感染，其在其宿主中诱导5种不同的效应，并感染25种昆虫和3种线虫。只有感染线虫的沃尔巴克氏体和赤眼蜂没有被噬菌体感染。所有25个检测到的病毒的特征是由一个小衣壳蛋白基因的DNA序列。基于目前可用的所有数据，系统发育分析显示，缺乏一致性之间的沃尔巴克氏体或昆虫和噬菌体WO基因，表明许多水平转移的噬菌体之间的不同沃尔巴克氏体菌株。噬菌体增殖和沃尔巴克氏体诱导的效果之间的关系的缺乏表明，WO是不直接参与这些效果。噬菌体WO进化的影响进行了讨论。
Bacteriophages are common viruses infecting prokaryotes. In addition to their deadly effect, phages are also involved in several evolutionary processes of bacteria, such as coding functional proteins potentially beneficial to them, or favoring horizontal gene transfer through transduction. The particular lifestyle of obligatory intracellular bacteria usually protects them from phage infection. However, Wolbachia, an intracellular alpha-proteobacterium, infecting diverse arthropod and nematode species and best known for the reproductive alterations it induces, harbors a phage named WO, which has recently been proven to be lytic. Here, phage infection was checked in 31 Wolbachia strains, which induce 5 different effects in their hosts and infect 25 insect species and 3 nematodes. Only the Wolbachia infecting nematodes and Trichogramma were found devoid of phage infection. All the 25 detected phages were characterized by the DNA sequence of a minor capsid protein gene. Based on all data currently available, phylogenetic analyses show a lack of congruency between Wolbachia or insect and phage WO phylogenies, indicating numerous horizontal transfers of phage among the different Wolbachia strains. The absence of relation between phage phylogeny and the effects induced by Wolbachia suggests that WO is not directly involved in these effects. Implications on phage WO evolution are discussed.