Comparative proteomic analysis reveals the mechanisms governing cotton fiber differentiation and initiation
Comparative proteomic analysis reveals the mechanisms governing cotton fiber differentiation and initiation
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DOI：
10.1016/j.jprot.2011.09.025
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发表时间：
2012-01-04
影响因子：
3.3
通讯作者：
Zhang, Tianzhen
中科院分区：
文献类型：
--
作者：
Liu, Kang;Han, Meiling;Zhang, Tianzhen
棉纤维是单细胞种子毛状体，为研究植物细胞模式提供了理想的模型。目前，纤维分化和起始的分子机制尚不清楚。为了确定参与这些过程的蛋白质，基于2-DE和MS/MS技术对无绒毛突变体（fl）与其亲本野生型（WT）进行了比较蛋白质组学分析。在花后3天和0天、棉纤维分化和起始期胚珠中分别鉴定出46个差异表达蛋白。大多数蛋白（72%）在fl突变体中的表达水平低于WT，这表明突变基因对胚珠发育有有害影响。用定量PCR检测蛋白水平和转录物水平的一致性。差异表达蛋白具有多种功能，涉及氧化还原稳态、应激反应、转录后和翻译后调控以及碳水化合物、能量、蛋白质、氨基酸和固醇代谢。在-3和-2 DPA胚珠表面检测到强烈的ROS爆发，并且在0 DPA下，fl突变体的几种碳水化合物浓度低于WT胚珠。这些发现表明，活性氧稳态可能是棉纤维形态发生的中心调控机制，转录后和翻译后调控可能在这一过程中起关键作用。(C) 2011 Elsevier B.V.版权所有
Cotton fibers are single-celled seed trichomes and provide an ideal model for studying plant cell patterning. At present, the molecular mechanisms of fiber differentiation and initiation are unclear. To determine the proteins involved in these processes, a comparative proteomic analysis based on 2-DE and MS/MS technology was conducted between the fuzzless-lintless mutant (fl) and its parental wild type (WT). Forty-six differentially expressed proteins were identified in ovules at -3 and 0 days post-anthesis (DPA), at the times of cotton fiber differentiation and initiation, respectively. Most of the proteins (72%) were expressed at lower levels in the fl mutant than in the WT, suggesting deleterious effects of the mutant genes on ovule development. The consistency between protein levels and transcript levels was examined using quantitative PCR. The differentially expressed proteins had diverse putative functions related to redox homeostasis, stress responses, post-transcriptional and post-translational regulation, and carbohydrate, energy, protein, amino acid, and sterol metabolism. A strong burst of ROS was detected on the surface of -3 and -2 DPA fl ovules, and the concentrations of several carbohydrates at 0 DPA were lower in the fl mutant than in the WT ovules. These findings suggest that ROS homeostasis may be a central regulatory mechanism for cotton fiber morphogenesis and that post-transcriptional and post-translational regulation may be pivotal in this process. (C) 2011 Elsevier B.V. All rights reserved.