Angiotensin II receptor subtypes in the rat brain.
Angiotensin II receptor subtypes in the rat brain.
复制标题
大鼠大脑中的血管紧张素 II 受体亚型。
DOI：
10.1016/0014-2999(90)90457-h
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发表时间：
1990
影响因子：
5
通讯作者：
Speth,RC
中科院分区：
文献类型：
--
作者：
Rowe,BP;Grove,KL;Saylor,DL;Speth,RC
非肽血管紧张素II(AII)受体亚型选择性拮抗剂DUP 753用于研究大鼠脑内AII受体的结合部位。DUP753在许多脑核(IC50=20−30 nM)竞争结合125I-[Sar1，Ile8]AII(125I-SIAII)，但在其余部位(IC50和10−4M)是一个弱竞争者。DUP753敏感结合位点(指定为AIIα亚型)对应于结合被巯基还原剂抑制的区域，而DUP753不敏感结合位点(AIIβ)对应于结合不被巯基还原剂抑制的区域。
The non-peptide angiotensin II (AII) receptor subtype selective antagonist, DuP 753, was used to characterize AII receptor binding sites in the rat brain. DuP 753 competed for specific125I-[Sar1, Ile8]AII (125I-SIAII) binding in many brain nuclei (IC50= 20−30 nM), but was a weak competitor at remaining sites (IC50> 10−4M). DuP 753 sensitive binding sites (designated AIIαsubtype) correspond with areas where binding is inhibited by sulfhydryl reducing agents, whereas DuP 753 insensitive sites (AIIβ) correspond with areas where binding is not inhibited by sulfhydryl reducing agents.