Identification of T-cell epitopes in nonstructural proteins of foot-and-mouth disease virus
Identification of T-cell epitopes in nonstructural proteins of foot-and-mouth disease virus
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DOI：
10.1128/jvi.75.7.3164-3174.2001
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发表时间：
2001-04-01
影响因子：
5.4
通讯作者：
Sobrino, F
中科院分区：
文献类型：
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作者：
Blanco, E;Garcia-Briones, M;Sobrino, F
使用体外淋巴细胞增殖反应测试了猪 T 细胞对口蹄疫病毒 (FMDV) 非结构蛋白 (NSP) 的识别。从实验性感染 FMDV 的远交猪中获得淋巴细胞。在不同的 NSP 中，多肽 3A、3B 和 3C 在体外测定中给出最高的刺激。使用重叠的合成肽可以识别这些蛋白质内被淋巴细胞有效识别的氨基酸区域。其中一些抗原肽的序列在不同的 FMDV 血清型中高度保守，它们引发了感染 C 型病毒或再次感染异源 FMDV 的猪的淋巴细胞的主要组织相容性复合物限制性反应，含有 T 细胞肽 3A[21-35] 和 B 细胞抗原位点 VP1 [137-156] 的串联肽也有效地刺激了感染的淋巴细胞体外动物。此外，这种串联肽引发了显着水平的血清型特异性抗病毒活性，这一结果与抗口蹄疫病毒抗体的诱导一致。因此，在肽制剂中包含源自具有诱导T辅助活性能力的NSP 3A的T细胞表位可以允许B细胞协同诱导抗FMDV抗体。
Porcine T-cell recognition of foot-and-mouth disease virus (FMDV) nonstructural proteins (NSP) was tested using in vitro lymphoproliferative responses, Lymphocytes were obtained from outbred pigs experimentally infected with FMDV, Of the different NSP, polypeptides 3A, 3B, and 3C gave the highest stimulations in the in vitro assays. The use of overlapping synthetic peptides allowed the identification of amino acid regions within these proteins that were efficiently recognized by the lymphocytes. The sequences of some of these antigenic peptides were highly conserved among different FMDV serotypes, They elicited major histocompatibility complex-restricted responses with lymphocytes from pigs infected with either a type C virus or reinfected with a heterologous FMDV, A tandem peptide containing the T-cell peptide 3A[21-35] and the B-cell antigenic site VP1 [137-156] also efficiently stimulated lymphocytes from infected animals in vitro. Furthermore, this tandem peptide elicited significant levels of serotype-specific antiviral activity, a result consistent with the induction of anti-FMDV antibodies. Thus, inclusion in the peptide formulation of a T-cell epitope derived from the NSP 3A possessing the capacity to induce T helper activity can allow cooperative induction of anti-FMDV antibodies by B cells.