Antimelanogenesis effect of Tunisian herb Thymelaea hirsuta extract on B16 murine melanoma cells
Antimelanogenesis effect of Tunisian herb Thymelaea hirsuta extract on B16 murine melanoma cells
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DOI：
10.1111/j.1600-0625.2007.00618.x
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发表时间：
2007-12-01
影响因子：
3.6
通讯作者：
Isoda, Hiroko
中科院分区：
文献类型：
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作者：
Kawano, Mitsuko;Matsuyama, Kyoko;Isoda, Hiroko
皮肤色素沉着是发生在黑素细胞和/或黑色素瘤细胞中的黑素生成的结果。尽管黑素生成对于防止紫外线辐射引起的DNA损伤和癌症是必要的，但黑色素的过度积累也会导致黑色素瘤。因此，我们重点研究了突尼斯草本植物Thymelaea hirsuta的提取物的抗黑色素瘤作用。以小鼠黑色素瘤B16细胞为研究对象，观察其对黑色素瘤B16细胞的生长抑制作用。我们发现毛毛藤提取物可降低B16细胞合成黑素的含量，且无细胞毒性。酪氨酸酶是黑素合成的关键酶，细胞外信号调节激酶(ERK)-1/2的磷酸化与黑素合成抑制有关。为了阐明其机制，我们还检测了ERK1/2的磷酸化和酪氨酸酶的表达水平。ERK1/2在用提取物处理后立即在细胞中被磷酸化。经24 h刺激后，酪氨酸酶的表达受到抑制。对毛毛瑞香提取物进行了分离，我们发现其中一个组分显著减少了B16细胞的黑色素合成，并且这个组分含有瑞香烷作为主要成分。这表明我们的发现可能归因于瑞香烷。
Skin pigmentation is the result of melanogenesis that occurs in melanocytes and/or melanoma cells. Although melanogenesis is necessary for the prevention of DNA damage and cancer caused by UV irradiation, excessive accumulation of melanin can also cause melanoma. Thus, we focused on the antimelanogenesis effect of an extract from Thymelaea hirsuta, a Tunisian herb. Murine melanoma B16 cells were treated with T. hirsuta extract, and then cell viability and synthesized melanin content were measured. We found that the T. hirsuta extract decreased the synthesized melanin content in B16 cells without cytotoxicity. Tyrosinase is a key enzyme of melanogenesis and extracellular signal-regulated kinase (ERK)-1/2 phosphorylation is known to be related to melanogenesis inhibition. To clarify its mechanism, we also determined ERK1/2 phosphorylation and tyrosinase expression level. ERK1/2 was immediately phosphorylated in cells just after treatment with the extract. The tyrosinase expression was inhibited after 24 h of stimulation with the extract. The T. hirsuta extract was fractionated, and we found that one fraction considerably decreased the melanin synthesis in B16 cells and that this fraction contains daphnanes as the main component. This indicates that our findings might be attributable to daphnanes.