A microdialysis study of the medial prefrontal cortex of adolescent and adult rats.
A microdialysis study of the medial prefrontal cortex of adolescent and adult rats.
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DOI：
10.1016/j.neuropharm.2011.04.005
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发表时间：
2011-09
影响因子：
4.7
通讯作者：
Mokler DJ
中科院分区：
文献类型：
--
作者：
Staiti AM;Morgane PJ;Galler JR;Grivetti JY;Bass DC;Mokler DJ
大鼠的内侧前额叶皮层（mPFC）已成为旨在阐明涉及注意和决策的行为基础（即执行功能）的研究的关键焦点。青少年mPFC是特别感兴趣的mPFC冲动和注意力的作用，以及注意力缺陷障碍等疾病。在本研究中，我们研究了基础细胞外浓度的神经递质5-羟色胺（5-HT），多巴胺（DA）和去甲肾上腺素（NE）的腹侧部分的mPFC（vmPFC）在青少年（出生后45-50天）和成年，男性和女性大鼠使用体内微透析。我们还检查了左和右VMPFC，给出了半球之间功能的偏侧性报告。5-HT的基础细胞外浓度在雄性和雌性大鼠之间存在显著差异。细胞外DA也显着不同的雄性和雌性大鼠之间和成年男性的左和右VMPFC。基础细胞外NE无差异。在右侧和左侧vmPFC之间的细胞外NE水平的偏侧性方面，组间存在显著的年龄差异。通过透析探针输注100 µM甲基苯丙胺增加了所有单胺的细胞外浓度，尽管甲基苯丙胺刺激释放的组间无差异。本研究结果表明，大鼠mPFC的单胺能输入存在年龄、性别和半球差异。这项工作为进一步研究发育过程中的前额叶皮层奠定了神经化学基础。
The medial prefrontal cortex (mPFC) of the rat has become a key focus of studies designed to elucidate the basis of behavior involving attention and decision making, i.e. executive functions. The adolescent mPFC is of particular interest given the role of the mPFC in impulsivity and attention, and disorders such as attentional deficit disorder. In the present study we have examined the basal extracellular concentrations of the neurotransmitters 5-hydroxytryptamine (5-HT), dopamine (DA) and norepinephrine (NE) in the ventral portion of the mPFC (vmPFC) in both adolescent (post-natal day 45–50) and adult, and male and female rats using in vivo microdialysis. We have also examined both the left and right vmPFCs given reports of laterality in function between the hemispheres. Basal extracellular concentrations of 5-HT differed significantly between male and female rats. Extracellular DA also differed significantly between male and female rats and between the left and the right vmPFC in adult males. No differences were seen in basal extracellular NE. There was a significant age difference between groups in the laterality of extracellular NE levels between right and left vmPFC. Infusion of 100 µM methamphetamine through the dialysis probe increased the extracellular concentration of all the monoamines although there were no differences between groups in methamphetamine stimulated release. The findings from this study demonstrate that there are differences in monoaminergic input to the mPFC of the rat based on age, gender and hemisphere. This work sets the neurochemical baseline for further investigations of the prefrontal cortex during development.