THE GLUCANASE-SOLUBLE MANNOPROTEINS LIMIT CELL-WALL POROSITY IN SACCHAROMYCES-CEREVISIAE
THE GLUCANASE-SOLUBLE MANNOPROTEINS LIMIT CELL-WALL POROSITY IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1002/yea.320060606
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发表时间：
1990-11-01
期刊：
影响因子：
2.6
通讯作者：
VANDENENDE, H
中科院分区：
文献类型：
--
作者：
DENOBEL, JG;KLIS, FM;VANDENENDE, H
分批培养的酿酒酵母细胞孔隙率在指数期早期最大，生长后期迅速下降到较低水平，用α-甘露糖苷酶处理后，细胞壁孔率恢复到指数期早期的水平。当用α-甘露糖苷酶或衣霉素(一种N-糖基化抑制剂)处理处于指数早期的细胞时，甚至可以获得更高的孔隙率。壁蛋白中含有截短甘露聚糖侧链的突变体也具有非常多孔的壁层。在性诱导过程中，甘露聚糖侧链对壁孔率的重要性也被发现。用阿尔法信息素处理，导致壁蛋白的形成具有较短的甘露聚糖侧链，增强了壁孔。二硫键也影响细胞壁的孔隙度。它们主要存在于葡聚糖酶可溶的壁蛋白中。由于甘露聚糖侧链的主要部分也存在于这个壁蛋白家族中，我们的结果表明，葡聚糖酶可溶的甘露糖蛋白限制了酵母细胞壁的孔隙度。
The cell porosity of batch‐grownSaccharomyces cerevisiaewas maximal in the early exponential phase and fell off rapidly to lower levels in later growth phases.Treatment of stationary‐phase cells with alpha‐mannosidase restored wall porosity to the level of cells in early exponential phase. When cells in the early exponential phase were treated with alpha‐mannosidase, or tunicamycin, an inhibitor of N‐glycosylation, even higher porosities were obtained. Mutants with truncated mannan side‐chains in their wall proteins also had very porous walls. The importance of the mannan side‐chains for wall porosity was also seen during sexual induction. Treatment with alpha pheromone, which leads to the formation of wall proteins with shorter mannan side‐chains, enhanced wall porosity.Disulphide bridges also affect cell wall porosity. They were predominantly found in the glucanase‐soluble wall proteins. Because the main part of the mannan side‐chains is also found in this family of wall proteins, our results demonstrate that the glucanase‐soluble mannoproteins limit cell wall porosity in yeast.