Niche segregation of ammonia-oxidizing archaea and anammox bacteria in the Arabian Sea oxygen minimum zone
Niche segregation of ammonia-oxidizing archaea and anammox bacteria in the Arabian Sea oxygen minimum zone
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DOI：
10.1038/ismej.2011.60
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发表时间：
2011-12-01
期刊：
影响因子：
11
通讯作者：
Damste, Jaap S. Sinninghe
中科院分区：
文献类型：
--
作者：
Pitcher, Angela;Villanueva, Laura;Damste, Jaap S. Sinninghe
氨氧化古菌（AOA）和厌氧氨氧化（anammox）细菌已成为海洋氮循环中的重要因素，分别负责将铵氧化为亚硝酸盐和二氮气体。这些群体之间存在互动的潜力；然而，它们的分布很少同时确定。在这里，我们使用完整极性脂质 (IPL) 和基于基因的分析的独特组合，在 DNA 和 RNA 水平上检查了 AOA 和厌氧氨氧化细菌在阿拉伯海最低氧区 (OMZ) 的垂直分布，阿拉伯海最低氧区是全球海洋中最强烈和垂直夸张的 OMZ 之一。为了筛选 AOA 特异性 IPL，我们开发了一种针对己糖-磷酸己糖 (HPH) crenarchaeol（培养 AOA 的常见 IPL）的高效液相色谱/质谱/质谱方法。 HPH-crenarchaeol 在氧浓度约为 1 时在上部 OMZ 过渡区显示出最高丰度。 5 mu M，与奇古菌 16S rDNA 和 amoA 基因丰度和基因表达的峰值一致。相比之下，厌氧氨氧化特异性 IPL 的浓度在 OMZ 核心 600 m 处达到峰值，此时氧气达到最低浓度，并且与厌氧氨氧化 16S rDNA 和肼氧化还原酶 (hzo) 基因丰度及其表达的峰值一致。总而言之，这些数据揭示了丰富的 AOA 和厌氧氨氧化细菌的独特深度分布以及它们各自生态位的隔离 > 400 m，表明它们的代谢在阿拉伯海 OMZ 采样的时间和地点没有直接耦合。 ISME 杂志 (2011) 5, 1896-1904; doi：10.1038/ismej.2011.60； 2011 年 5 月 19 日在线发布
Ammonia-oxidizing archaea (AOA) and anaerobic ammonia-oxidizing (anammox) bacteria have emerged as significant factors in the marine nitrogen cycle and are responsible for the oxidation of ammonium to nitrite and dinitrogen gas, respectively. Potential for an interaction between these groups exists; however, their distributions are rarely determined in tandem. Here we have examined the vertical distribution of AOA and anammox bacteria through the Arabian Sea oxygen minimum zone (OMZ), one of the most intense and vertically exaggerated OMZs in the global ocean, using a unique combination of intact polar lipid (IPL) and gene-based analyses, at both DNA and RNA levels. To screen for AOA-specific IPLs, we developed a high-performance liquid chromatography/mass spectrometry/mass spectrometry method targeting hexose-phosphohexose (HPH) crenarchaeol, a common IPL of cultivated AOA. HPH-crenarchaeol showed highest abundances in the upper OMZ transition zone at oxygen concentrations of ca. 5 mu M, coincident with peaks in both thaumarchaeotal 16S rDNA and amoA gene abundances and gene expression. In contrast, concentrations of anammox-specific IPLs peaked within the core of the OMZ at 600 m, where oxygen reached the lowest concentrations, and coincided with peak anammox 16S rDNA and the hydrazine oxidoreductase (hzo) gene abundances and their expression. Taken together, the data reveal a unique depth distribution of abundant AOA and anammox bacteria and the segregation of their respective niches by >400 m, suggesting no direct coupling of their metabolisms at the time and site of sampling in the Arabian Sea OMZ. The ISME Journal (2011) 5, 1896-1904; doi: 10.1038/ismej.2011.60; published online 19 May 2011