Comprehensive investigation of p53, p21, nm23, and VEGF expression in hepatitis B virus-related hepatocellular carcinoma overall survival after hepatectomy
Comprehensive investigation of p53, p21, nm23, and VEGF expression in hepatitis B virus-related hepatocellular carcinoma overall survival after hepatectomy
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全面调查p53、p21、nm23和VEGF表达在乙型肝炎病毒相关性肝细胞癌肝切除术后总生存中的影响。
DOI：
10.7150/jca.33766
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发表时间：
2020-01
影响因子：
3.9
通讯作者：
Ye X
中科院分区：
文献类型：
--
作者：
Zhu Guang-Zhi;Liao Xi-Wen;Wang Xiang-Kun;Gong Yi-Zhen;Liu Xiao-Guang;Yu Long;Han Chuang-Ye;Yang Cheng-Kun;Su Hao;Huang Ke-Tuan;Yu Ting-Dong;Huang Jian-Lu;Li Jia;Zeng Zhi-Ming;Qin Wei;Liu Zheng-Qian;Zhou Xin;Liu Jun-Qi;Lu Lei;Han Quan-Fa;Shang Li-Ming;Ye X
目的：本研究旨在探讨p53、p21、nm 23和VEGF在B型肝炎病毒（HBV）相关性肝癌（HCC）切除术后预后中的免疫组化表达及其作为预后指标的分子机制。研究方法：对419例广西南部地区HBV相关性肝癌患者进行免疫组化检测，以评估这些生物标志物的表达与预后的关系。从GSE 14520获得HBV相关HCC的全基因组表达微阵列数据集。结果如下：在我们的研究中，p53、p21和nm 23在乙型肝炎相关肝细胞癌患者癌组织中的表达不影响2年、5年或总体的临床结局。VEGF高表达患者术后2年总生存率低于低表达患者（校正P=0.040，校正HR = 1.652，95% CI = 1.024-2.665）。GSE 14520队列中VEGF的生存分析也表明VEGF mRNA表达与HBV相关的HCC OS显著相关（校正P=0.035，校正HR =1.651，95% CI =1.035-2.634）。共表达分析提示VEGF可能参与调控细胞增殖、生长和凋亡过程，参与调控Rap 1信号通路、HIF-1信号通路、PPAR信号通路、细胞周期。而GSEA提示VEGF可能参与了HIF和HIF 1A通路的调节，以及TP 53调节通路。结论：我们的研究结果表明，VEGF可能是HBV相关HCC的预后指标，我们还通过全基因组共表达和GSEA方法确定了VEGF的潜在分子机制。
Objective: The goal of our current study is to assess the immunohistochemical of p53, p21, nm23, and VEGF expression in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) prognosis after hepatectomy, as well as the prospective molecular mechanisms of prognostic indicator. Methods: There were 419 HBV-related HCC patients who were from southern China of Guangxi province and were used to evaluate the immunohistochemical expression for these biomarkers in prognosis. A genome-wide expression microarray dataset of HBV-related HCC were obtained from GSE14520. Results: In our study, the expression of p53, p21, and nm23 in cancer tissues of patients with hepatitis B-related hepatocellular carcinoma did not affected the clinical outcome of 2 years, 5 years or overall. Patients with high expression of VEGF had a worse overall survival after 2 years of surgery than patients with low expression (adjusted P=0.040, adjusted HR = 1.652, 95% CI = 1.024-2.665). Survival analysis of VEGF in GSE14520 cohort also demonstrated that VEGF mRNA expression also significantly associated with HBV-related HCC OS (adjusted P=0.035, adjusted HR =1.651, 95% CI =1.035-2.634). The prospective molecular mechanisms by co-expression analysis suggested that VEGF might be correlated to regulation of cell proliferation, cell growth and apoptotic process, Rap1 signaling pathway, HIF-1 signaling pathway, PPAR signaling pathway, cell cycle. Whereas the GSEA suggested that VEGF might involve in the regulation of HIF and HIF1A pathway, and TP53 regulation pathway. Conclusion: Our findings suggested that VEGF might be a prognostic indicator of HBV-related HCC, and we also identified the VEGF prospective molecular mechanisms through the whole genome co-expression and GSEA approaches.
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影响因子：
3.9
作者：
Liao X;Liu X;Yang C;Wang X;Yu T;Han C;Huang K;Zhu G;Su H;Qin W;Huang R;Yu L;Deng J;Zeng X;Ye X;Peng T
通讯作者：
Peng T
DOI：
10.4321/s1130-01082012000600003
发表时间：
2012-06-01
期刊：
Revista Española de Enfermedades Digestivas
影响因子：
--
作者：
Gómez-Rodríguez, Rafael;Romero-Gutiérrez, Marta;Sánchez-Ruano, Juan José
通讯作者：
Sánchez-Ruano, Juan José
影响因子：
29.4
作者：
H. Gerdes
通讯作者：
H. Gerdes
影响因子：
3.5
作者：
An, Run;Meng, Jie;Dong, Xiao-Ping
通讯作者：
Dong, Xiao-Ping
瘤周肝细胞和基质细胞之间的串扰在涉及瘤周 VEGF-C、VEGFR-1 和 VEGFR-3 的肝细胞癌中的预后作用
影响因子：
3.7
作者：
Zhuang PY;Shen J;Zhu XD;Lu L;Wang L;Tang ZY;Sun HC
通讯作者：
Sun HC