Chemokine mRNA expression in gastric mucosa is associated with Helicobacter pylori cagA positivity and severity of gastritis
Chemokine mRNA expression in gastric mucosa is associated with Helicobacter pylori cagA positivity and severity of gastritis
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DOI：
10.1136/jcp.51.10.765
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发表时间：
1998-10-01
影响因子：
3.4
通讯作者：
Crabtree, JE
中科院分区：
文献类型：
--
作者：
Shimoyama, T;Everett, SM;Crabtree, JE
目的：探讨幽门螺杆菌相关性胃炎组织中胃趋化因子mRNA表达水平与胃炎严重程度及cagA阳性之间的关系。方法：对83例消化不良患者胃窦及胃体活检标本进行半定量逆转录聚合酶链式反应(RT-PCR)检测，并与胃炎组织学分级进行比较。胃炎评分采用视觉模拟评分。RT-PCR扩增趋化因子IL-8、Groα、ENA-78、RANTES、MCP-1，并与G3PDH扩增产物进行比较。结果5种趋化因子在幽门螺杆菌阳性的粘膜中的表达均显著高于幽门螺杆菌阴性的粘膜。在幽门螺杆菌阳性患者中，cagA阳性患者胃窦部C-X-C趋化因子mRNA表达显著高于cagA阴性患者，但C-C趋化因子mRNA表达差异无统计学意义。在幽门螺杆菌阳性患者中，趋化因子在胃体部的表达低于胃窦部。与胃窦部相比，cagA阳性组仅GroA基因的表达显著增强。中性粒细胞浸润与C-X-C趋化因子mRNA表达相关。结论C-X-C趋化因子在活动性胃炎中可能起主要作用。CagA阳性幽门螺杆菌感染可诱导胃窦粘膜趋化因子mRNA表达增加，这可能与cagA阳性幽门螺杆菌感染引起的胃黏膜损伤增加有关。
Aim-To investigate the association between the quantity of gastric chemokine mRNA expression, severity of gastritis, and cagA positivity in Helicobacter pylori associated gastritis.Methods-In 83 dyspeptic patients, antral and corpus biopsies were taken for semiquantitative reverse transcription polymerase chain reaction (RT-PCR) and histological grading of gastritis. Gastritis was evaluated by visual analogue scales. Quantities of chemokine (IL-8, GRO alpha, ENA-78, RANTES, MCP-1) RT-PCR products were compared with G3PDH products. Each sample was also evaluated for the presence of cagA and ureA mRNA by RT-PCR.Results-mRNA expression of all five chemokines was significantly greater in H pylori positive than in H pylori negative mucosa. In H pylori positive patients, in the antrum C-X-C chemokine mRNA expression was significantly greater in cagA positive patients than in cagA negative patients, but there were no significant differences in C-C chemokine mRNA expression. In H pylori positive patients, chemokine mRNA expression in the corpus was less than in the antrum. In contrast to the antrum, only GROa mRNA expression was significantly greater in cagA positive infection. Polymorphonuclear cell infiltration was correlated with C-X-C chemokine mRNA expression. Significant correlations were also found between bacterial density and C-X-C chemokine mRNA expression.Conclusions-In H pylori infection, C-X-C chemokines may play a primary role in active gastritis. Infection with cagA positive H pylori induces greater gastric chemokine mRNA expression in the antral mucosa, which may be relevant to the increased mucosal damage associated with cagA positive H pylori infection.