Potential for phosphonoacetate utilization by marine bacteria in temperate coastal waters
Potential for phosphonoacetate utilization by marine bacteria in temperate coastal waters
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DOI：
10.1111/j.1462-2920.2008.01745.x
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发表时间：
2009-01-01
影响因子：
5.1
通讯作者：
Joint, Ian
中科院分区：
文献类型：
--
作者：
Gilbert, Jack A.;Thomas, Simon;Joint, Ian
磷酸盐是一种含有C-P键的有机化合物，是海洋磷循环中特征不明显的组成部分。它们可能是生物可利用磷的潜在来源，特别是在缺营养条件下。本研究研究了编码磷酸酯水解酶的phnA基因的分布，磷酸酯水解酶是矿化磷酸酯的酶。利用新设计的针对phnA基因的简并引物，我们分析了从2006年7月西英吉利海峡浮游植物爆发中构建的DNA和cDNA文库中磷乙酸利用的潜力。从cDNA中分离总RNA并进行逆转录，用退化引物PCR扩增出磷酸乙酸水解酶（phnA）转录物，克隆并测序。系统发育分析表明，14种序列类型具有相当大的多样性，产生5种独特的phnA蛋白群。在一项来自沿海海洋中游环境的454焦磷酸测序宏基因组和亚转录组研究中，我们还鉴定出28个phnA同源物，表明本研究中>.3 %的海洋细菌含有phnA。phnA同源物也存在于本实验的宏基因组化石文库中。最后，四株属于弧菌科的潜在珊瑚病原体的培养物含有phnA基因。在实验室中，这些菌株能够以磷酸醋酸酯作为唯一的磷和碳源生长。三种沿海环境对醋酸磷的利用能力都很明显，这表明这种生物可利用磷源具有广泛利用的潜力。
Phosphonates are organic compounds that contain a C-P bond and are a poorly characterized component of the marine phosphorus cycle. They may represent a potential source of bioavailable phosphorus, particularly in oligotrophic conditions. This study has investigated the distribution of the phnA gene which encodes phosphonoacetate hydrolase, the enzyme that mineralizes phosphonoacetate. Using newly designed degenerate primers targeting the phnA gene we analysed the potential for phosphonoacetate utilization in DNA and cDNA libraries constructed from a phytoplankton bloom in the Western English Channel during July 2006. Total RNA was isolated and reverse transcribed and phosphonoacetate hydrolase (phnA) transcripts were PCR amplified from the cDNA with the degenerate primers, cloned and sequenced. Phylogenetic analysis demonstrated considerable diversity with 14 sequence types yielding five unique phnA protein groups. We also identified 28 phnA homologues in a 454-pyrosequencing metagenomic and metatranscriptomic study from a coastal marine mesocosm, indicating that > 3% of marine bacteria in this study contained phnA. phnA homologues were also present in a metagenomic fosmid library from this experiment. Finally, cultures of four isolates of potential coral pathogens belonging to the Vibrionaceae contained the phnA gene. In the laboratory, these isolates were able to grow with phosphonoacetate as sole P and C source. The fact that the capacity to utilize phosphonoacetate was evident in each of the three coastal environments suggests the potential for widespread utilization of this bioavailable P source.