Advances in tissue engineering of vasculature through three-dimensional bioprinting.
Advances in tissue engineering of vasculature through three-dimensional bioprinting.
复制标题
通过三维生物打印在脉管系统组织工程方面取得进展。
DOI：
10.1002/dvdy.385
复制
发表时间：
2021
影响因子：
2.5
通讯作者：
Wang Jian
中科院分区：
文献类型：
--
作者：
Zhu Junjin;Wang Yuting;Zhong Linna;Pan Fangwei;Wang Jian
背景组织工程学面临的一个重大挑战是构建血管构造物，它包含血管组织构造物以概括可存活的、复杂的和有功能的器官或组织，以及未来可能提供临床应用的独立的血管结构。三维生物打印已经成为一种很有前途的技术，具有许多其他传统生物制造方法所不具备的优点。在过去的十年中，3D生物打印已经贡献了多种技术和策略来生成血管组织结构和独立的血管结构。结果本文重点介绍了两种血管结构的打印策略，即血管组织结构和血管样管结构，强调了当前血管结构构建方法的可行性和不足。通常，直接打印和间接打印都可用于血管组织工程。直接打印允许通过同步细胞播种进行结构制造，而间接打印在生成复杂结构方面更有效。在制作过程中，应有选择地采用挤压生物打印、喷墨生物打印和光辅助生物打印等三维生物打印技术，以发挥其优势，获得理想的组织结构。此外，合适的细胞和生物材料对于匹配各种生物打印技术，从而实现特定血管结构的成功制造至关重要。结论3D生物打印技术的发展有助于提供各种制造技术，致力于生产结构稳定、生理相关和具有生物吸引力的结构。然而，尽管生物材料的优化和打印策略的创新可能会改善所制造的脉管状结构，但3D生物打印仍处于婴儿期，离体外实验和体内应用还有很大的差距。本文综述了三维生物打印在构建血管构建物方面的研究进展，并对未来先进血管构造物的构建方向进行了展望。
BackgroundA significant challenge facing tissue engineering is the fabrication of vasculature constructs which contains vascularized tissue constructs to recapitulate viable, complex and functional organs or tissues, and free‐standing vascular structures potentially providing clinical applications in the future. Three‐dimensional (3D) bioprinting has emerged as a promising technology, possessing a number of merits that other conventional biofabrication methods do not have. Over the last decade, 3D bioprinting has contributed a variety of techniques and strategies to generate both vascularized tissue constructs and free‐standing vascular structures.ResultsThis review focuses on different strategies to print two kinds of vasculature constructs, namely vascularized tissue constructs and vessel‐like tubular structures, highlighting the feasibility and shortcoming of the current methods for vasculature constructs fabrication. Generally, both direct printing and indirect printing can be employed in vascularized tissue engineering. Direct printing allows for structural fabrication with synchronous cell seeding, while indirect printing is more effective in generating complex architecture. During the fabrication process, 3D bioprinting techniques including extrusion bioprinting, inkjet bioprinting and light‐assisted bioprinting should be selectively implemented to exert advantages and obtain the desirable tissue structure. Also, appropriate cells and biomaterials matter a lot to match various bioprinting techniques and thus achieve successful fabrication of specific vasculature constructs.ConclusionThe 3D bioprinting has been developed to help provide various fabrication techniques, devoting to producing structurally stable, physiologically relevant, and biologically appealing constructs. However, although the optimization of biomaterials and innovation of printing strategies may improve the fabricated vessel‐like structures, 3D bioprinting is still in the infant period and has a great gap between in vitro trials and in vivo applications. The article reviews the present achievement of 3D bioprinting in generating vasculature constructs and also provides perspectives on future directions of advanced vasculature constructs fabrication.