Sequence Analysis of Inducible Prophage phIS3501 Integrated into the Haemolysin II Gene of Bacillus thuringiensis var israelensis ATCC35646.
Sequence Analysis of Inducible Prophage phIS3501 Integrated into the Haemolysin II Gene of Bacillus thuringiensis var israelensis ATCC35646.
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DOI：
10.1155/2012/543286
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发表时间：
2012
影响因子：
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通讯作者：
Sorokin A
中科院分区：
文献类型：
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作者：
Moumen B;Nguen-The C;Sorokin A
蜡状芽孢杆菌属细菌引起的腹泻性食物中毒主要是由于基因组中编码的几种毒素所致。其中一种是细胞毒素K，最近被发现与严重的坏死性综合征有关。细胞毒素K与蜡样芽孢杆菌大多数基因组中通常被称为溶血素II(HlyII)的基因编码的一类蛋白质相似。苏云金芽孢杆菌以色列变种ATCC35646的基因组部分序列含有几个潜在的诱导前驱体，其中一个整合到hlyII基因中。我们测定了这个原噬菌体命名为phIS3501的完整序列，并建立了基因组组织。在丝裂霉素C诱导切除该原噬菌体的过程中，形成了完整的hlyII基因，从而为细胞提供了合成活性毒素的遗传能力。因此，这种原噬菌体在被切除后，可能与活性毒素的合成调节有关，从而与细菌宿主的毒力有关。这种遗传排列与金黄色葡萄球菌 β-溶血素的遗传排列相似，表明了细菌病原体对这种系统的选择的一般性。
Diarrheic food poisoning by bacteria of the Bacillus cereus group is mostly due to several toxins encoded in the genomes. One of them, cytotoxin K, was recently identified as responsible for severe necrotic syndromes. Cytotoxin K is similar to a class of proteins encoded by genes usually annotated as haemolysin II (hlyII) in the majority of genomes of the B. cereus group. The partially sequenced genome of Bacillus thuringiensis var israelensis ATCC35646 contains several potentially induced prophages, one of them integrated into the hlyII gene. We determined the complete sequence and established the genomic organization of this prophage-designated phIS3501. During induction of excision of this prophage with mitomycin C, intact hlyII gene is formed, thus providing to cells a genetic ability to synthesize the active toxin. Therefore, this prophage, upon its excision, can be implicated in the regulation of synthesis of the active toxin and thus in the virulence of bacterial host. A generality of selection for such systems in bacterial pathogens is indicated by the similarity of this genetic arrangement to that of Staphylococcus aureus β-haemolysin.