Separation and detection of bis(alkylthio)selenides of penicillamine and glutathione by liquid chromatography with electrochemical detection.
Separation and detection of bis(alkylthio)selenides of penicillamine and glutathione by liquid chromatography with electrochemical detection.
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液相色谱电化学检测法分离和检测青霉胺和谷胱甘肽的双（烷硫基）硒化物。
DOI：
10.1016/s0021-9673(01)92673-1
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Rabenstein,DL
中科院分区：
文献类型：
--
作者：
Killa,HM;Rabenstein,DL
描述了同时测定亚硒酸与硫醇反应形成的混合物中硫醇、二硫化物和双（烷硫基）硒化物的灵敏和选择性方法。该方法基于通过反相离子对色谱分离各种化合物，然后使用在洗脱液流中串联配备双汞/金汞齐电极的电化学检测器进行检测。直接在下游电极处检测硫醇。双（烷硫基）硒化物和二硫化物的间接检测方法是首先在上游电极将其还原，然后在下游电极检测还原产物。双（烷硫基）硒化物在比二硫化物更低的负电势下被还原，使得可以在与二硫化物的混合物中选择性地检测它们。检测器对双（青霉胺）硒化物的响应从 1.1 · 10-7Mup 到至少 1.2 · 10-4M 呈线性；检测限估计为 < 2 pmol 双（青霉胺）硒化物。
Sensitive and selective methodology is described for the simultaneous determination of thiols, disulfides and bis(alkylthio)selenides in mixtures formed by reaction of selenious acid with thiols. The methods are based on separation of the various compounds by reversed-phase ion pairing chromatography followed by detection with an electrochemical detector equipped with dual mercury/gold amalgam electrodes in series in the eluent stream. Thiols are detected directly at the downstream electrode. Bis(alkylthio)selenides and disulfides are detected indirectly by first reducing them at the upstream electrode followed by detection of the reduction products at the downstream electrode. Bis(alkylthio)selenides are reduced at less negative potentials than disulfides, making it possible to selectively detect them in mixtures with disulfides. Detector response to bis(penicillamine)selenides is linear from 1.1 · 10-7Mup to at least 1.2 · 10-4M; the detection limit is estimated to be < 2 pmol bis(penicillamine)selenide.