State-dependent pontine ensemble dynamics and interactions with cortex across sleep states
State-dependent pontine ensemble dynamics and interactions with cortex across sleep states
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DOI：
10.1101/752683
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发表时间：
2019-09
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影响因子：
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通讯作者：
Tomomi Tsunematsu;Amisha A. Patel;A. Onken;S. Sakata
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文献类型：
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作者：
Tomomi Tsunematsu;Amisha A. Patel;A. Onken;S. Sakata
脑桥核在睡眠-觉醒调节中起着至关重要的作用。然而，脑桥整体动态睡眠调节的基础仍然知之甚少。通过监测多个脑桥和邻近脑干区域的群体活动，在这里，我们显示了小鼠脑桥和皮质之间缓慢的、状态预测的脑桥整体动力学和状态依赖的相互作用。在几秒到几分钟的时间尺度上，脑桥群体在警惕状态下表现出不同的放电，其中一些动态归因于细胞类型特异性活动。脑桥群体活动可以预测瞳孔扩张和警觉状态：脑桥神经元表现出较长的预测能力相比，海马神经元。在亚秒的时间尺度上，脑桥波（P波）被观察为脑桥神经元的同步放电，主要在快速眼动（REM）睡眠期间，但也在非REM（NREM）睡眠期间。重要的是，P波在功能上以状态依赖的方式与皮层活动相互作用：在NREM睡眠期间，海马尖波波纹（SWR）先于P波。另一方面，快速眼动睡眠期间的P波与正在进行的海马θ振荡锁相，并且随后在海马神经元的子集中爆发放电。因此，海马体和脑桥之间的功能相互作用的方向性取决于睡眠状态。脑桥和皮层区域之间的这种状态依赖性全局协调暗示了睡眠的不同功能作用。
The pontine nuclei play a crucial role in sleep-wake regulation. However, pontine ensemble dynamics underlying sleep regulation remain poorly understood. By monitoring population activity in multiple pontine and adjacent brainstem areas, here we show slow, state-predictive pontine ensemble dynamics and state-dependent interactions between the pons and the cortex in mice. On a timescale of seconds to minutes, pontine populations exhibit diverse firing across vigilance states, with some of these dynamics being attributed to cell type-specific activity. Pontine population activity can predict pupil dilation and vigilance states: pontine neurons exhibit longer predictable power compared with hippocampal neurons. On a timescale of sub-seconds, pontine waves (P-waves) are observed as synchronous firing of pontine neurons primarily during rapid eye movement (REM) sleep, but also during non-REM (NREM) sleep. Crucially, P-waves functionally interact with cortical activity in a state-dependent manner: during NREM sleep, hippocampal sharp wave-ripples (SWRs) precede P-waves. On the other hand, P-waves during REM sleep are phase-locked with ongoing hippocampal theta oscillations and are followed by burst firing in a subset of hippocampal neurons. Thus, the directionality of functional interactions between the hippocampus and pons changes depending on sleep states. This state-dependent global coordination between pontine and cortical regions implicates distinct functional roles of sleep.