Fibroblast attachment onto novel titanium mesh membranes for guided bone regeneration
Fibroblast attachment onto novel titanium mesh membranes for guided bone regeneration
复制标题
DOI：
10.1007/s10266-014-0151-8
复制
发表时间：
2015-05-01
期刊：
影响因子：
2.5
通讯作者：
Koyano, Kiyoshi
中科院分区：
文献类型：
--
作者：
Rakhmatia, Yunia Dwi;Ayukawa, Yasunori;Koyano, Kiyoshi
钛网在骨科手术中被用作屏障膜，因为它具有合适的特性，可以在新骨形成期间提供机械支持。一个理想的膜将有助于细胞附着在其表面，从而有助于稳定血栓并将膜整合到组织中。然而，目前可用的钛网孔尺寸为毫米级，这导致软组织通过毛孔生长。因此，本研究的目的是研究成纤维细胞在不同设计的微米级微孔钛网上的附着和迁移情况。本研究使用了六种新型钛网膜和三组市售钛网膜。从4日龄绿色荧光蛋白大鼠体内分离出成纤维细胞，接种于膜表面。培养24 h后，固定贴壁细胞，DAPI染色。计数膜表面及上下两侧蓝色染色的细胞核。结果表明，不同的膜材料、结构和设计在细胞附着到膜表面的能力上有很大的差异。新型膜，尤其是12孔的膜与多孔的膜相比，允许成纤维细胞附着在膜表面，但阻碍了成纤维细胞通过孔向膜下侧的迁移，这与临床上的缺损区有关。
Titanium mesh is used in orthopedic surgery as a barrier membrane, as it offers suitable characteristics, which allow mechanical support during the formation of new bone. An ideal membrane would facilitate cell attachment onto its surface, thereby helping to stabilize the blood clot and integrate the membrane into the tissue. However, currently available titanium mesh has millimeter-level pore sizes, which lead to soft tissue ingrowth through the pores. Therefore, the aim of this study was to investigate the fibroblast attachment and migration on different designs of novel titanium mesh with micrometer pore size for guided bone regeneration treatment. Six types of novel titanium mesh membrane and three groups of commercially available membranes were used in this study. Fibroblasts were isolated from 4-day-old green fluorescence protein rats and seeded onto membrane surfaces. At 24 h, the cells attached to the membrane surfaces were fixed and stained with DAPI. The blue-stained nuclei on membrane surfaces, and both upper and lower sides were counted. It was shown that different membrane materials, structure and design differ considerably in their capacity for cell attachment to the membrane surface. The novel membranes, especially mesh with 12 pores compared with mesh with multi-pores, allowed the fibroblast attachment on the membrane surface, but hindered the fibroblast migration through the pores into the lower side of the membrane, which is associated with the defect area in the clinical condition.