INDUCED TRANSPOSITION OF DS BY A STABLE AC IN CROSSES OF TRANSGENIC TOBACCO PLANTS
INDUCED TRANSPOSITION OF DS BY A STABLE AC IN CROSSES OF TRANSGENIC TOBACCO PLANTS
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DOI：
10.1007/bf00330942
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发表时间：
1989-05-01
期刊：
影响因子：
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通讯作者：
BAKER, B
中科院分区：
文献类型：
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作者：
HEHL, R;BAKER, B
在转基因烟草中建立了一个双组分玉米转座子系统，该系统包含一个稳定但反式活性的ac元件和一个顺式响应的ds元件。该系统的开发对于实现在异源植物物种中使用这些元件的基因标签策略是可取的。已鉴定出含有1个Ac转座元件(Ac-18)的转基因烟草，该元件末端有4个碱基对缺失。稳定的Ac-18转座酶活性在组织培养实验中和转基因植株中都得到了证实。当含有Ac-18基因的植株与含有ds基因的转基因植株杂交时，25%-50%的F1代表现出DS转座的反式激活。对几个F1植株的DNA分析表明，每个植株都表现出独特的DS转座到新的染色体位置的模式。
A two component maize transposon system comprising a stable but trans-active Ac element and a cis responsive Ds element has been established in transgenic tobacco [Nicotiana tabacum). The development of this system is desirable for the realization of a gene tagging strategy employing these elements in heterologous plant species. Transgenic tobacco with a single transposed Ac element (Ac-18) which has sustained a 4 bp terminal deletion has been identified. Transposase activity of the stable Ac-18 was demonstrated in a tissue culture assay as well as in transgenic plants. When plants containing Ac-18 were crossed with transgenic plants carrying a Ds element, 25%-50% of the F1 progeny showed trans-activation of Ds transposition. Analysis of DNA of several F1 plants showed that each plant displayed a unique pattern of Ds transposition to new chromosomal sites.