Animal model - Histologic, serologic, and molecular analysis of persistent ehrlichiosis in a murine model
Animal model - Histologic, serologic, and molecular analysis of persistent ehrlichiosis in a murine model
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DOI：
10.1016/s0002-9440(10)63361-5
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发表时间：
2004-09-01
影响因子：
6
通讯作者：
Walker, DH
中科院分区：
文献类型：
--
作者：
Olano, JP;Wen, G;Walker, DH
1987年报道了由查菲埃立克体引起的人类嗜单核细胞埃立克体病，在小鼠中研究急性致死性埃立克体病的动物模型已经被开发为与人类嗜单核细胞埃立克体病的致死性形式非常相似。然而，免疫活性小鼠中埃立克体属持续感染的动物模型尚未得到表征。我们报告了免疫活性小鼠（AKR和C57 BL/6株）中小鼠埃立克体感染的组织病理学进展，其抗体反应通过间接免疫荧光和Western免疫印迹法测定，埃立克体负荷的分布和数量通过免疫组织化学，聚合酶链反应（PCR）和实时PCR在肺，肝和脾中。在这些器官的组织病理学分级和相对埃里希体负荷之间观察到轻度至中度相关性。最高的埃利希亚尔。在感染后第4至14天之间存在负载。感染后150天，通过实时PCR检测到组织中的小鼠肠杆菌。血清学反应的分析揭示了几种免疫显性抗原，包括200-、180-、100-、73/75-、45-和28-kd蛋白。总之，我们第一次提供了一个完整的组织病理学，血清学，免疫组化和定量分析的动物模型的持续埃立克体感染的研究。
Human monocytotropic ehrlichiosis caused by Ehrlichia chaffeensis was reported in 1987, An animal model to study acute fatal ehrlichiosis In mice that has been developed closely resembles the fatal form of human monocytotropic ehrlichiosis. However, animal models for persistent infection in the genus Ehrlichia in immunocompetent mice have not been characterized. We report the histopathological progression of Ehrlichia muris infection in immunocompetent mice (AKR and C57BL/6 strains) correlated with their antibody response determined by indirect immunofluorescence and Western immunoblotting, and the distribution and quantity of the ehrlichial load by immunohistochemistry, polymerase chain reaction (PCR), and real-time PCR in lungs, liver, and spleen. Mild to moderate correlation was observed between histopathological grading in these organs and relative ehrlichial loads. The highest ehrlichial. loads were present between days 4 and 14 after infection. E muris was detected in tissues examined up to 150 days after infection by real-time PCR. Analysis of the serological response revealed several immunodominant antigens, including 200-, 180-, 100-, 73/75-, 45-, and 28-kd proteins. In conclusion, we have provided for the first time a complete histopathological, serological, immunohistochemical, and quantitative analysis of an animal model for the study of persistent ehrlichial infection.