NORMAL OXIDATIVE DAMAGE TO MITOCHONDRIAL AND NUCLEAR-DNA IS EXTENSIVE
NORMAL OXIDATIVE DAMAGE TO MITOCHONDRIAL AND NUCLEAR-DNA IS EXTENSIVE
复制标题
DOI：
10.1073/pnas.85.17.6465
复制
发表时间：
1988-09-01
影响因子：
11.1
通讯作者：
AMES, BN
中科院分区：
文献类型：
--
作者：
RICHTER, C;PARK, JW;AMES, BN
DNA的氧化损伤可由辐射产生的激发氧或有氧代谢的副产物引起。氧化碱，8-羟基脱氧鸟苷（oh8dG）， 1 .apprxeq。在大鼠肝脏DNA中检测了20种已知的辐射损伤产物。oh8dG在核DNA中每13万个碱基中有1个，在mtDNA中每8000个碱基中有1个。用各种促氧化剂处理的线粒体的oh8dG水平增加。mtDNA中oh8dG的高水平可能与线粒体中巨大的氧代谢、相对低效的DNA修复和缺乏组蛋白有关。这可能是观察到的mtDNA高突变率的原因。
Oxidative damage to DNA can be caused by excited oxygen species, which are produced by radiation or are by-products of aerobic metabolism. The oxidized base, 8-hydroxydeoxyguanosine (oh8dG), 1 of .apprxeq. 20 known radiation damage products, has been assayed in the DNA of rat liver. oh8dG is present at a level of 1 per 130,000 bases in nuclear DNA and 1 per 8000 bases in mtDNA. Mitochondria treated with various prooxidants have an increased level of oh8dG. The high level of oh8dG in mtDNA may be caused by the immense oxygen metabolism, relatively inefficient DNA repair, and the absence of histones in mitochondria. It may be responsible for the observed high mutation rate of mtDNA.