Regions of hepatitis C virus E2 required for membrane association.
Regions of hepatitis C virus E2 required for membrane association.
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膜关联所需的丙型肝炎病毒E2区域。
DOI：
10.1038/s41467-023-36183-y
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发表时间：
2023-01-26
影响因子：
16.6
通讯作者：
Marcotrigiano, Joseph
中科院分区：
文献类型：
--
作者：
Kumar, Ashish;Rohe, Tiana C.;Elrod, Elizabeth J.;Khan, Abdul G.;Dearborn, Altaira D.;Kissinger, Ryan;Grakoui, Arash;Marcotrigiano, Joseph
Hepatitis C virus (HCV) uses a hybrid entry mechanism. Current structural data suggest that upon exposure to low pH and Cluster of Differentiation 81 (CD81), the amino terminus of envelope glycoprotein E2 becomes ordered and releases an internal loop with two invariant aromatic residues into the host membrane. Here, we present the structure of an amino-terminally truncated E2 with the membrane binding loop in a bent conformation and the aromatic side chains sequestered. Comparison with three previously reported E2 structures with the same Fab indicates that this internal loop is flexible, and that local context influences the exposure of hydrophobic residues. Biochemical assays show that the amino-terminally truncated E2 lacks the baseline membrane-binding capacity of the E2 ectodomain. Thus, the amino terminal region is a critical determinant for both CD81 and membrane interaction. These results provide new insights into the HCV entry mechanism. Hepatitis C virus (HCV) uses a hybrid entry mechanism. Upon exposure to low pH, envelope glycoprotein E2 releases an internal loop into the host membrane. Here we show the amino terminal region is a critical determinant for membrane interaction, providing insights into the HCV entry mechanism.
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影响因子：
64.8
作者：
Kumar A;Hossain RA;Yost SA;Bu W;Wang Y;Dearborn AD;Grakoui A;Cohen JI;Marcotrigiano J
通讯作者：
Marcotrigiano J
影响因子：
6.7
作者：
Krey T;d'Alayer J;Kikuti CM;Saulnier A;Damier-Piolle L;Petitpas I;Johansson DX;Tawar RG;Baron B;Robert B;England P;Persson MA;Martin A;Rey FA
通讯作者：
Rey FA
影响因子：
3.6
作者：
Axley P;Ahmed Z;Ravi S;Singal AK
通讯作者：
Singal AK
影响因子：
7.7
作者：
Flyak, Andrew, I;Ruiz, Stormy E.;Bjorkman, Pamela J.
通讯作者：
Bjorkman, Pamela J.
影响因子：
16.6
作者：
Janus BM;van Dyk N;Zhao X;Howell KA;Soto C;Aman MJ;Li Y;Fuerst TR;Ofek G
通讯作者：
Ofek G