Reactivity of the monocyte/macrophage system to superparamagnetic anionic nanoparticles
Reactivity of the monocyte/macrophage system to superparamagnetic anionic nanoparticles
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DOI：
10.1039/b903306h
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发表时间：
2009-01-01
影响因子：
--
通讯作者：
Wilhelm, Claire
中科院分区：
文献类型：
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作者：
Luciani, Nathalie;Gazeau, Florence;Wilhelm, Claire
纳米级磁性材料越来越多地应用于生物医学领域，如医学成像、药物输送、磁性分选和治疗性热疗。特别是，磁性细胞标记为MRI细胞成像提供了有吸引力的可能性，允许在活体动物中跟踪细胞群。因此，了解纳米颗粒与细胞的相互作用是至关重要的。在这里，我们描述了THP1单核细胞和衍生巨噬细胞对阴离子磁性纳米颗粒的摄取。通过电子显微镜观察纳米颗粒的掺入情况。然后定量细胞捕获作为孵育时间和细胞外铁的函数。一个简单的结合内化机制可以模拟所有的摄取曲线，从而得到亲和常数、可以在细胞膜上吸附的最大质量、内化时间常数和容量。虽然单核细胞和巨噬细胞的吸附步骤相当，但后者的内吞活性高出十倍以上。对于这两种细胞类型，我们报告了极好的磁标记效率，单核细胞中最大负载6个pgFe细胞（-1），衍生巨噬细胞中最大负载近50个pgFe细胞（-1）。最后，我们证明了内吞纳米颗粒不会影响标记单核细胞向巨噬细胞的分化。
Nano-scale magnetic materials are increasingly being used in the biomedical field for medical imaging, drug-delivery, magnetic sorting, and therapeutic hyperthermia. In particular, magnetic cell labelling offer attractive possibilities for MRI cell imaging, allowing the tracking of a cell population in living animals. Understanding of the interaction of nanoparticles with cells is then of paramount importance. Here, we characterize the uptake of anionic magnetic nanoparticles by THP1 monocytes and derived macrophages. The incorporation of nanoparticles was assessed by electron microscopy. Cell capture was then quantitated as a function of incubation time and extracellular iron. A simple binding internalisation mechanism allowed modelling all uptake curves, leading to the affinity constants, the maximal mass that can adsorb on the cell membrane, the internalisation time constants and capacities. Though the adsorption step was comparable for monocytes and macrophages, the latter exhibited a more than ten times higher endocytotic activity. For both cell types, we report an excellent efficiency of the magnetic label, with a maximum load of 6 pgFe cell(-1) in monocytes and almost 50 pgFe cell(-1) in derived macrophages. Finally, we demonstrated that endocytosed nanoparticles did not affect differentiation of labelled monocytes into macrophages.