Sequence features surrounding the translation initiation sites assigned on the genome sequence of Synechocystis sp. strain PCC6803 by amino-terminal protein sequencing.
Sequence features surrounding the translation initiation sites assigned on the genome sequence of Synechocystis sp. strain PCC6803 by amino-terminal protein sequencing.
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DOI：
10.1093/dnares/3.4.225
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发表时间：
1996-08-31
期刊：
影响因子：
--
通讯作者：
Ohara, O
中科院分区：
文献类型：
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作者：
Sazuka, T;Ohara, O
为了研究集胞藻6803株基因组翻译起始位点周围的序列特征，采用双向电泳技术对细胞总蛋白进行解析，并对相对丰富的蛋白质点进行氨基端序列测定。通过将确定的氨基末端序列与整个基因组的核苷酸序列进行比较，成功地在基因组上分配了总共72种蛋白质的翻译起始位点。翻译起始位点及其周围的核苷酸序列具有以下特点：（1）除了ATG、GTG和TTG三个起始密码子外，ATT也是一个罕见的起始密码子;（2）核心序列在35个起始位点之前的适当位置鉴定了Shine-Dalgarno序列的三个核苷酸（GAGG、GGAG和AGGA（48.6%）;和（3）起始密码子周围的优先序列用公式表示为5 '-YY[...]其中Y和R分别表示嘧啶和嘌呤核苷酸，三个点表示起始密码子。本研究的结果为基因发现软件的改进提供了有价值的信息，同时本研究所采用的方法也适用于细胞蛋白质表达谱的综合分析。
To characterize the sequence features surrounding the translation initiation sites on the genome of Synechocystis sp. strain 6803, the total proteins extracted from the cell were resolved by two-dimensional electrophoresis, and the amino-terminal sequences of the relatively abundant protein spots were determined. By comparison of the determined amino-terminal sequences with the nucleotide sequence of the entire genome, the translation initiation sites of a total of 72 proteins were successfully assigned on the genome. The sequence features emerged from the nucleotide sequences at and surrounding the translation initiation sites were as follows: (1) In addition to the three initiation codons, ATG, GTG, and TTG, evidence was obtained that ATT was also used as a rare initiation codon; (2) the core sequences (GAGG, GGAG and AGGA) of the Shine-Dalgarno sequence were identified in the appropriate position preceding the 35 initiation sites (48.6%); and (3) the preferential sequence surrounding the initiation codons was formulated as 5'-YY[...]R-3' where Y and R denote pyrimidine and purine nucleotides, respectively, and three dots represent the initiation codons. The result obtained would provide valuable information for improvement of the gene-finding software, and the approach used in this study should be applicable for comprehensive analysis of the expression profiles of cellular proteins.