Tissue-engineered bone repair of goat femur defects with osteogenically induced bone marrow stromal cells
Tissue-engineered bone repair of goat femur defects with osteogenically induced bone marrow stromal cells
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DOI：
10.1089/ten.2006.12.423
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发表时间：
2006-03-01
影响因子：
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通讯作者：
Cao, YL
中科院分区：
文献类型：
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作者：
Zhu, L;Liu, W;Cao, YL
组织工程技术可以产生骨组织，并已被证明是一种更好的手段来修复负重骨缺损。然而，以前的研究，迄今为止，已被限制到使用非成骨诱导的骨髓基质细胞（BMSCs）或缓慢降解支架的应用。在这项研究中，承重骨工程成骨诱导骨髓基质细胞。此外，珊瑚被用作支架材料，由于其适当的降解速率的工程和修复山羊股骨缺损。在10只山羊的右侧股骨中部创建一个25 mm长的缺损。缺损修复率进行了比较，在实验组的10只山羊接受植入物含有成骨诱导的BMSCs和对照组的山羊（n = 10）只接受珊瑚柱。实验组4个月时大体观察和X线片观察均见骨愈合，8个月时工程骨进一步改建为新形成的皮质骨。修复区X线片灰度值增加，与对照组比较有显著性差异（P < 0.05）。H&E染色显示骨小梁在4个月时形成。此外，在8个月时观察到不规则的骨单位。最重要的是，在弯曲载荷强度和弯曲刚度方面，组织工程骨段显示与左侧正常股骨相似，显示无显著差异（p > 0.05）。相反，对照组的珊瑚圆柱体显示没有骨形成。此外，载体几乎完全吸收，在对照组中在2个月时明显。H&E染色显示，4个月时少量残留的珊瑚颗粒被纤维组织包围，而8个月时残留物消失。基于这些结果，我们得出结论，从成骨诱导的骨髓基质干细胞和珊瑚工程骨可以理想地愈合关键大小的节段性骨缺损的山羊负重区。
Tissue engineering can generate bone tissue and has been shown to provide a better means of repairing weight-bearing bone defect. Previous studies, however, have heretofore been limited to the use of nonosteogenically induced bone marrow stromal cells (BMSCs) or the application of slow-degradation scaffolds. In this study, weight-bearing bone was engineered using osteogenically induced BMSCs. In addition, coral was used as a scaffold material, due to its proper degradation rate for the engineering and repair of a goat femur defect. A 25 mm long defect was created at the middle of the right femur in each of 10 goats. The rates of defect repair were compared in an experimental group of ten goats receiving implants containing osteogenically induced BMSCs and in the control group of goats (n = 10) receiving just coral cylinders. In the experimental group, bony union was observed by radiographic and gross view at 4 months, and engineered bone was further remodeled into newly formed cortexed bone at 8 months. There was increased gray density of radiographic rays in the repaired area, which was significantly different ( p < 0.05) from that of the control group. H&E staining demonstrated that trabecular bone was formed at 4 months. Moreover, irregular osteon was observed at 8 months. Most importantly, the tissue-engineered bone segment revealed a similarity to the left-side normal femur in terms of bend load strength and bend rigidity, showing no significant difference ( p > 0.05). In contrast, the coral cylinders of the control group showed no bone formation. Furthermore, almost complete resorption of the carrier had occurred, being evident at 2 months in the control group. H&E staining demonstrated that a small amount of residual coral particle was surrounded by fibrous tissue at 4 months whereas the residues disappeared at 8 months. Based on these results, we conclude that engineered bone from osteogenically induced BMSCs and coral can ideally heal critical-sized segmental bone defects in the weight-bearing area of goats.