Adiponectin resides in mouse skin and upregulates hyaluronan synthesis in dermal fibroblasts
Adiponectin resides in mouse skin and upregulates hyaluronan synthesis in dermal fibroblasts
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DOI：
10.3109/03008207.2010.528566
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发表时间：
2011-08-01
影响因子：
2.9
通讯作者：
Inoue, Shintaro
中科院分区：
文献类型：
--
作者：
Akazawa, Yumiko;Sayo, Tetsuya;Inoue, Shintaro
脂肪组织是一种具有生殖活性的组织，其产生影响其他组织活性的脂肪因子。脂联素是一种脂肪细胞特异性脂肪因子，参与全身代谢。检测脂联素受体（AdipoR 1和AdipoR 2）mRNA在培养的真皮成纤维细胞中的表达。全长脂联素（fAd），而不是球状脂联素（gAd），增加透明质酸（HA）的生产和上调HA合成酶（HAS）2 mRNA的表达。AdipoR 1和AdipoR 2 mRNA也在角质形成细胞中表达，尽管fAd和gAd对HA合成都没有任何影响。在小鼠皮肤中，我们发现脂联素是存在的，并随着年龄的增长显着下降。脂联素在皮肤中随年龄的增加而降低，与皮肤中HA的降低有很好的相关性。我们的实验也是第一个确定脂联素生产培养的小鼠皮脂腺细胞，这一发现表明，皮肤脂联素可能不仅来自血浆和/或皮下脂肪组织，但也从皮脂腺。提示脂联素在皮肤HA代谢中起重要作用。
Adipose tissue is a hormonally active tissue that produces adipokines that influence the activity of other tissues. Adiponectin is an adipocyte-specific adipokine involved in systemic metabolism. We detected the expression of adiponectin receptors (AdipoR1 and AdipoR2) mRNA in cultured dermal fibroblasts. The full-length adiponectin (fAd), but not the globular adiponectin (gAd), increased hyaluronan (HA) production and upregulated HA synthase (HAS) 2 mRNA expression. AdipoR1 and AdipoR2 mRNAs were also expressed in keratinocytes, though neither fAd nor gAd had any effect on HA synthesis. In mouse skin, we found that adiponectin was present and decreased markedly with aging. The age-dependent pattern of adiponectin decrease in skin, correlated well with that of HA in skin. Our experiments were also the first to identify adiponectin production in cultured mouse sebocytes, a finding that suggests that skin adiponectin may derive not only from plasma and/or subcutaneous adipose tissue, but also from the sebaceous gland. These results indicated that adiponectin plays an important role in the HA metabolism of skin.