Sox17-2A-iCre: A Knock-in Mouse Line Expressing Cre Recombinase in Endoderm and Vascular Endothelial Cells
Sox17-2A-iCre: A Knock-in Mouse Line Expressing Cre Recombinase in Endoderm and Vascular Endothelial Cells
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DOI：
10.1002/dvg.20540
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发表时间：
2009-09-01
期刊：
影响因子：
1.5
通讯作者：
Lickert, Heiko
中科院分区：
文献类型：
--
作者：
Engert, Silvia;Liao, W. Perry;Lickert, Heiko
Sox17编码一种与SRY相关的高迁移率族（HMG）盒转录因子，该因子对内胚层形成和胎儿造血干细胞的维持至关重要。在小鼠中，首先在胚外内胚层中观察到Sox17的表达，随后在定形内胚层以及发育中的血管的血液和内皮细胞中也能看到其表达。为了在这些区域有条件地使基因失活，我们将Sox17基因座作为靶点，以产生一种双顺反子mRNA，它通过病毒2A序列将Sox17与一种密码子优化的Cre重组酶（iCre）连接起来。在此我们报道一种新的Cre敲入小鼠品系，Sox17 - 2A - iCre，其在发育中的内胚层、心血管系统的血管内皮细胞以及造血系统中具有活性。我们的研究结果表明，Sox17 - 2A - iCre在早期内胚层祖细胞中具有活性，并且在Sox17所有先前报道的表达区域中都观察到了Rosa26报告基因的重组。Sox17 - 2A - iCre品系将是在定形内胚层以及血管和造血系统中有条件地使基因失活的极佳工具。《发生》47:603 - 610, 2009。（C）2009威利 - 利斯公司
Sox17 encodes an SRY-related high-mobility group (HIVIG) box transcription factor that is essential for endoderm formation and fetal hematopoietic stem cell maintenance. In the mouse, expression of Sox17 is first observed in the extraembryonic endoderm and is subsequently seen in the definitive endoderm as well as in blood and the endothelial cells of the developing vasculature. To conditionally inactivate genes in these domains, we have targeted the Sox17 locus to generate a bicistronic mRNA linking Sox17 to a codon improved Cre recombinase (iCre) via a viral 2A sequence. Here we report a new Cre knock-in mouse line, Sox17-2A-iCre, with activity in the developing endoderm, the vascular endothelial cells of the cardiovascular system and the hematopoietic system. Our results indicate that the Sox17-2A-iCre is active in an early endoderm progenitor and recombination of the Rosa26 reporter was observed in all previously reported expression domains of Sox17. The Sox17-2A-iCre line will be an excellent tool to conditionally inactivate genes in the definitive endoderm as well as in the vasculature and hematopoietic system. genesis 47:603-610, 2009. (C) 2009 Wiley-Liss, Inc.